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C2c12 mouse premyoblasts

C2C12 mouse premyoblasts are a well-established cell line derived from mouse skeletal muscle. They are capable of differentiating into mature muscle cells and are commonly used for the study of myogenesis and muscle development.

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2 protocols using c2c12 mouse premyoblasts

1

C2C12 Myoblast Differentiation Assay

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All reagents were purchased from Sigma-Aldrich and used without any further purification unless otherwise indicated. C2C12 mouse premyoblasts and Dulbecco's modified Eagle's medium (DMEM) were obtained from ATCC. Foetal bovine serum (FBS), penicillin/streptomycin (P/S), phosphate buffered saline (PBS), Modified Eagle's Medium (MEM), Hank's Balanced Salt Solution (HBSS) were ordered from Gibco® (Life Technologies). Normal goat serum, modified nucleotide 5-ethynyl-2′-deoxyuridine (EdU), 4',6-diamidino-2-phenylindole HCl (DAPI) and all Alexa Fluor® conjugated products for immunofluorescence staining were ordered from Life Technologies. Sodium alginate was purchased from FMC Biopolymer.
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2

C2C12 Myoblast Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were purchased from Sigma-Aldrich and used without any further purification unless otherwise indicated. C2C12 mouse premyoblasts and Dulbecco's modified Eagle's medium (DMEM) were obtained from ATCC. Foetal bovine serum (FBS), penicillin/streptomycin (P/S), phosphate buffered saline (PBS), Modified Eagle's Medium (MEM), Hank's Balanced Salt Solution (HBSS) were ordered from Gibco® (Life Technologies). Normal goat serum, modified nucleotide 5-ethynyl-2′-deoxyuridine (EdU), 4',6-diamidino-2-phenylindole HCl (DAPI) and all Alexa Fluor® conjugated products for immunofluorescence staining were ordered from Life Technologies. Sodium alginate was purchased from FMC Biopolymer.
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