Summit version 4.3

Manufactured by Agilent Technologies

Summit (version 4.3) is a versatile lab equipment product from Agilent Technologies. It is designed to perform essential laboratory functions. The product's core function is to provide reliable and efficient data analysis capabilities for researchers and scientists.

Automatically generated - may contain errors

Lab products found in correlation

T25 tissue culture flask, by Corning (1 mentions) Click it edu alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Fxcycle violet, by Thermo Fisher Scientific (1 mentions) Hypotonic pi solution, by Merck Group (1 mentions) Facscalibur cytometer, by BD (1 mentions)

Spelling variants of this product

Summit 4.3 software (43 citations) Summit version 4.3 software (7 citations)

2 protocols using summit version 4.3

Melanoma Cell Cycle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Melanoma cells were grown to approximately 60% confluence in T25 tissue culture flasks (Corning Product #430639). Cells were labeled with Click-iT EdU Alexa fluor 488 (C10425, Invitrogen) followed by detection using FxCycle Violet (F-10347, Invitrogen), per manufacturer's recommendations. Data acquisition was accomplished using CyanADP from Bechman Coulter, and cell cycle analysis accomplished using Summit (version 4.3) software (DAKO).

Carson C.C., Moschos S.J., Edmiston S.N., Darr D.B., Nikolaishvili-Feinberg N., Groben P.A., Zhou X., Kuan P.F., Pandey S., Chan K.T., Jordan J.L., Hao H., Frank J.S., Hopkinson D.A., Gibbs D.C., Alldredge V.D., Parrish E., Hanna S.C., Berkowitz P., Rubenstein D.S., Miller C.R., Bear J.E., Ollila D.W., Sharpless N.E., Conway K, & Thomas N.E. (2015). IL-2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(9), 2167-2176.

+ Open protocol

+ Expand

Radiation-Induced Cell Cycle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

FaDu cells (200,000 cells/flask) were seeded in T‐25 flasks. The following day the flasks were treated with 1 μM AZD5363, and 2 h later, 4 Gy RT was delivered to the cells at a rate of 0.952 Gy/min (Faxitron). After 24 h, the contents of the flasks media were removed, centrifuged (400 g for 5 min), and the cell pellet was resuspended in 300 μl hypotonic PI solution (50 μg/ml PI (Sigma‐Aldrich), 0.1% (w/v) sodium citrate, 0.1% (v/v) Triton X‐100) and left overnight at 4°C. 10,000 events were then analysed using a FACSCalibur cytometer (BD Biosciences), and the data examined using the software Summit version 4.3 (Dako).

Searle E.J., Telfer B.A., Mukherjee D., Forster D.M., Davies B.R., Williams K.J., Stratford I.J, & Illidge T.M. (2017). Akt inhibition improves long‐term tumour control following radiotherapy by altering the microenvironment. EMBO Molecular Medicine, 9(12), 1646-1659.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!