PBMC samples were stained with the following antibodies: CD3-PerCP clone HIT3a, CD3-APC clone SK7, CD45-PerCP clone 2D1, NKG2D-PE clone 1D11, KIR2DL2/DL3-FITC clone DX27, NKp30-PE clone P30-15 (all Sony Biotechnology, San Jose, CA, USA); CD3-APC-Vio770 clone REA613, CD56-Vio-Blue clone REA196, CD45-Vio-Green clone 5B1, CD14-PE-Vio-770 TÜK4, HLA-DR-PE-Vio-770 clon AC122, CD57-APC-Vio-770 clon TB03, NKG2C-FITC clone REA205, NKG2A-PE/PE-Vio615 clone REA110, CD16-APC clone REA423 (all Miltenyi Biotec, Bergisch Gladbach, Germany); CD38 (Beckman Coulter, USA); PD-1-Alexa Fluor 647 (clone EH12.2H7, BioLegend, San Diego, CA, USA). Samples were analyzed using a MACSQuant 10 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) equipped with lasers λ = 405 nm, λ = 488 nm, λ = 635 nm; threshold was set to cut off events with low CD45 staining. The applied panels of fluorochrome-conjugated monoclonal antibodies to the listed immune cell surface markers are shown in Table 1 . Samples were analyzed using a MACSQuant 10 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) equipped with lasers λ = 405 nm, λ = 488 nm, and λ = 635 nm.
Pd 1 alexa fluor 647
Manufactured by BioLegend
Sourced in United States
PD-1-Alexa Fluor 647 is a fluorescently-labeled antibody that binds to the programmed cell death-1 (PD-1) protein. It is designed for use in flow cytometry and other immunology applications.
Automatically generated - may contain errors
Lab products found in correlation
Macsquant 10 flow cytometer, by Miltenyi Biotec (1 mentions)
Cd3 percp clone hit3a, by Sony (1 mentions)
Sytox blue dead cell stain, by Thermo Fisher Scientific (1 mentions)
Cd56 apc, by Beckman Coulter (1 mentions)
Cd45 vioblue, by Miltenyi Biotec (1 mentions)
Hla dr pe, by Sony (1 mentions)
Cd14 pe vio770, by Miltenyi Biotec (1 mentions)
Cd45ra apc, by Sony (1 mentions)
Cd197 fitc, by Sony (1 mentions)
Cd16 apc, by Miltenyi Biotec (1 mentions)
Cd56 apc vio770, by Miltenyi Biotec (1 mentions)
Cd3 vioblue, by Miltenyi Biotec (1 mentions)
2 protocols using pd 1 alexa fluor 647
1
Immunophenotyping of PBMC Samples
2
Comprehensive Immune Phenotyping of PBMC Samples
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PBMC samples were stained with the panels of fluorochrome-conjugated monoclonal antibodies, which are in the Supplementary Table S1 . The list of mouse anti-human antibodies included: NKG2C-FITC (4:100, clone REA205), CD3-Vioblue (0.4:100, clone BW264/56), CD56-APC-Vio770 (clone REA196), CD45-VioBlue (2:100, clone REA747), CD14-PE-Vio-770 (0.4:100, clone TÜK4), CD16-APC (3:100, clone REA423) from Miltenyi Biotec, Bergisch Gladbach, Germany; HLA-DR-PE (3:100, clone L243, Sony Biotechnology, San Jose, CA, USA), CD3-PerCP (5:100, clone HIT3a), CD3-FITC (3:100, clone FIT3a), CD45RA-APC (2:100, clone HI100), CD197-FITC (5:100, clone G043H7), KIR2DL2/L3- APC (1:100, clone DX27), NKp30-PE (3:100, clone P30-15), NKG2D-PE (3:100, clone 1D11), CD45-PerCP (2:100, clone 2D1), CD57-APC (4:100, clone HNK-1) from Sony Biotechnology, San Jose, CA, USA; CD56-APC (3:100, clone N901) from Beckman Coulter, Brea, CA, USA, PD-1-Alexa Fluor 647 (1:100, clone EH12.2H7) from BioLegend, San Diego, CA, USA.
Viability staining with SytoxBlue Dead Cell stain was performed (Invitrogen, Waltham, MA, USA). The gating strategy is presented inFigure 1 .
Viability staining with SytoxBlue Dead Cell stain was performed (Invitrogen, Waltham, MA, USA). The gating strategy is presented in
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