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Human ifn α

Manufactured by Roche
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Human IFN-α is a recombinant protein that represents the alpha subtypes of human interferon. Interferons are a group of signaling proteins released by host cells in response to the presence of several pathogens, such as viruses, bacteria, parasites, or tumor cells. The core function of human IFN-α is to induce cellular resistance to viral infection.

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Lab products found in correlation

Stat1, by BD (1 mentions) Ifn λ2, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Phospho stat1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

IFN-α (8 citations) Recombinant human IFN-α-2a (2 citations)

2 protocols using human ifn α

1

IFN-α Stimulation of Immune Cells

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HeLa and 293T cells were obtained from ATCC. Mouse bone marrow cells and splenocytes were cultured in RPMI 1640 with 10% fetal bovine serum (FBS). Cells were stimulated with 1,000 U/ml human IFN-α (Roche) overnight.
Stawowczyk M., Naseem S., Montoya V., Baker D.P., Konopka J, & Reich N.C. (2018). Pathogenic Effects of IFIT2 and Interferon-β during Fatal Systemic Candida albicans Infection. mBio, 9(2), e00365-18.
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2

Interferon Signaling in Cells

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Human IFN-α (Roferon-A) was a gift from Roche. IFN-λ2 was purchased from PeproTech, Inc. The following antibodies were used for Western blotting and immunofluorescence staining: phospho-STAT1 and phospho-STAT1 Alexa Fluor 555 conjugated (Cell Signaling Technology), STAT1 (BD), β-actin (Sigma-Aldrich), and IFN-λR1 (ProSci Inc.).
Duong F.H., Trincucci G., Boldanova T., Calabrese D., Campana B., Krol I., Durand S.C., Heydmann L., Zeisel M.B., Baumert T.F, & Heim M.H. (2014). IFN-λ receptor 1 expression is induced in chronic hepatitis C and correlates with the IFN-λ3 genotype and with nonresponsiveness to IFN-α therapies. The Journal of Experimental Medicine, 211(5), 857-868.
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