The morphology of ZnO nanostructures was investigated by field-emission electron microscopy (FE-SEM, Hitachi S-4700, Tokyo, Japan), optical properties (absorbance and bandgap) were investigated by using UV-Vis spectrophotometer (Perkin-Elmer Lambda 750) and structural study was done by X-ray diffractometer (XRD) (Rigaku, radiation Cu kα, λ = 1.5406 Å). Photoluminescence (PL) (LS-55 Luminescence, Perkin Elmer, Germany), and X-ray photoelectron spectroscopy (XPS) were used to investigate defect states in the nanostructures. To estimate photovoltage in the visible region, a mercury lamp (power 100 watt) was used as a light source, whose intensity on the surface of photodetectors was adjusted 1 mW cm−2. The photovoltage of fabricated photo-detectors was recorded at room temperature using digital multimeter. In the photo-detection experiments, bias voltage of 5 V was applied. UV diode and optical filters with transmittance wavelength in the range 380~420 nm, 490~560 nm and λ > 420 nm (white light) were used to select different spectrum regions, respectively. The ‘ON’ and ‘OFF’ state of incident radiations were controlled by using a camera shutter. Schematic layout of the experimental set-up for the measurement of photo-generated voltage is shown in Figure S1 .
Ls55 luminescence
Manufactured by PerkinElmer
Sourced in Germany, United Kingdom
The LS55 Luminescence Spectrometer is a high-performance analytical instrument designed for accurate and sensitive measurements of luminescence phenomena. It offers a range of features, including xenon flash lamp excitation, dual monochromators, and a photomultiplier tube detector, to provide precise spectral analysis and quantification of luminescent samples.
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4 protocols using ls55 luminescence
1
Characterization of ZnO Nanostructure Photovoltaic Properties
2
Spectroscopic Characterization of Metal Complexes
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Fourier transformed infrared spectra were collected in transmission mode using KBr pellet technique on a Perkin Elmer BX FTIR. Electronic spectra of complexes and ligands were recorded in DMSO (5 × 10 -5 M) using Perkin Elemer BioLambda 35 in 260-1100 nm range. Fluorimetric measurements were done on PerkinElmer LS55 Luminescence. Elemental analyses of C, H and N content were performed on a Perkin Elmer 2400 Series II CHNS analyzer. Copper content in complexes was determined by spectrophotometry using neocuproin method. 27 (link) Mass spectra were collected from DMSO solution of complexes using Shimadzu LCMS-2020. Conductometry measurements were done in DMSO solution using Phywe conductometer.
3
Thiazole Orange DNA Binding Assay
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FID titrations were performed on a Perkin Elmer LS55 Luminescence equipped with thermostated cell holder. Spectra were acquired by using an excitation wavelength of 501 nm and recording the signal in the 520-680 nm emission range at 25 °C. A solution containing 0.62 μM of target DNA and 1.24 μM of thiazole orange (TO) was prepared in a 10 mm path-length cell and the corresponding fluorescence spectrum was acquired in the absence and presence of increasing concentrations of tested compounds in 10 mM Tris, 50 mM KCl, pH 7.5. The percentage of TO displacement was calculated as TO displacement = 100 -[(F/F0) × 100], where F0 is the fluorescence in the absence of ligand and F the fluorescence recorded at each point of titration. TO displacement was plotted as a function of compound concentration and the EC50 (half maximal effective concentration) was calculated. Each titration was repeated at least in triplicate.
4
Tryptophan Fluorescence Titration of Protein
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Tryptophan fluorescence was measured on a LS-55 luminescence spectrometer (Perkin-Elmer Instruments, Chalfont St. Giles, UK), using excitation at 280 nm and emission at 300-450 nm and excitation/emission slits of 10/10 nm. The protein had an initial concentration of 0.015 mg/mL (1.1 μM) and was titrated with vitD. A total of 60 μL of vitD was added to the protein, diluting the protein by up to 4%. This was corrected in the final data.
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