An intraperitoneal glucose tolerance test (IPGTT) was performed on a subset of the total animals used (Female LH: n = 11; Female LH17LNa: n = 9; Male LH: n = 10; Male LH17LNa: n = 10) between 10 and 12 weeks of age. Rats were fasted for 6 h between 5AM and 11AM, before a baseline blood draw and glucose check via the tail vein. All animals were then given a (1 mg/kg) intraperitoneal dextrose injection (Cat#1046864; Henry Schein), and blood was sampled at regular intervals via tail vein for glucose (Contour Next, Cat#7278, Ascensia Bayer). Plasma was collected concurrently into tubes (Sarstedt Microvette Tubes with Heparin, Cat#NC9046728; Fisher Scientific) to measure insulin, according to the manufacturer’s specifications (Rat Insulin ELISA, Cat#80-INSRT-E01, ALPCO).
Rat insulin elisa
Manufactured by ALPCO
Sourced in United States
The Rat Insulin ELISA is a laboratory test kit used to measure the concentration of insulin in rat samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) designed to quantify insulin levels accurately and reliably.
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Lab products found in correlation
7 protocols using rat insulin elisa
1
Glucose Tolerance and Insulin Response in LH Rats
2
Rat Fasting Blood Glucose and Insulin
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Fasting blood samples were taken from conscious rats by lateral tail vein sampling for the assessment of blood glucose concentration (automatic blood glucose monitor, Contour, Bayer Consumer Care AG, Basel, Switzerland) and plasma insulin levels (Rat Insulin ELISA, Alpco, Salem, NH, USA).
3
Plasma Analysis of Metabolic Markers
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At termination, hearts were exsanguinated and blood in the thoracic cavity collected in K2‐EDTA tubes and centrifuged immediately for separation of plasma that was used for determination of insulin (rat insulin ELISA; ALPCO, Salem, NH), FA (NEFA‐HR; Wako Diagnostics, Mountain View, CA) and triglyceride (Stanbio Triglycerides LiquiColor Mono; Thermo Fisher Scientific, Waltham, MA). After acid‐ethanol extraction, insulin content in the pancreas was assessed by ELISA.
4
Metabolic Biomarkers in Kidney Physiology
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Serum insulin was measured by Rat Insulin ELISA (ALPCO Diagnostics, Salem, NH). Homeostasis model assessment insulin resistance (HOMA‐IR) was calculated by the following formula: HOMA‐IR=fasting blood glucose (mmol/L)×fasting insulin (μU/mL)/22.4. Serum Aldo and plasma corticosterone levels were determined using a solid‐phase radioimmunoassay (Diagnostic Products, Los Angeles, CA) and corticosterone 3H radioimmunoassay kit (MP Biomedicals, LLC, Solon, OH). Plasma renin activity (PRA) was measured by radioimmunoassay (DiaSorin, Stillwater, MN). Plasma adiponectin level was measured by ELISA (Rat Adiponectin, EMD Millipore, Darmstadt, Germany). Kidney NAD+ concentration was determined by NAD+/NADH Quantification Colorimetric Kit (BioVision, Milpitas, CA).
5
Fasting Rat Insulin and Glucose
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Fasting blood samples were taken from the lateral tail vein of conscious rats for determination of plasma insulin levels (Rat Insulin ELISA, Alpco, Salem, NH, USA) and blood glucose concentration (automatic blood glucose monitor, Contour, Bayer Consumer Care AG, Basel, Switzerland).
6
Plasma Biomarkers Evaluation in Rodents
7
Glucose-Stimulated Insulin Secretion Assay
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Naked islets and islets-laden microgels were first cultured in RPMI-1640 media with low glucose concentration (2.8 mM) for 30 min, then transferred into RPMI-1640 media with high glucose concentration (16.7 mM) for 30 min. The supernatants were collected at the end of each incubation and the process was repeated for 4 times incessantly. Quantification of insulin were determined via rat insulin enzyme-linked immunosorbent assay (Rat Insulin ELISA, ALPCO, NH, USA) according to the manufacturer's instructions. In addition, the stimulation index (insulin release at high/low glucose concentration) was calculated for both naked islets and islets-laden microgels.
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