Platinum taq hf

Manufactured by Thermo Fisher Scientific

Platinum Taq HF is a high-fidelity DNA polymerase enzyme used for PCR amplification. It provides efficient and accurate DNA replication with a low error rate.

Automatically generated - may contain errors

Lab products found in correlation

Platinum pfx, by Thermo Fisher Scientific (1 mentions) Dneasy dna extraction kit, by Qiagen (1 mentions) Dpni, by New England Biolabs (1 mentions)

Close spelling variants of this product

Platinum Taq (212 citations) Platinum Taq DNA Polymerase HF (3 citations) Platinum Taq-HF polymerase (2 citations)

2 protocols using platinum taq hf

Targeted CFTR Gene Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was isolated using DNeasy DNA extraction kit (Qiagen). A 2.6-kb PCR product was generated using Platinum pfx (Invitrogen) and primers FP-i9.1 and RP-i10.1. A second PCR was performed using sequencing labelled CFTR-NHEJ^For and CFTR-NHEJ^Rev primers to produce a 432 bp product which was subjected to next generation sequencing analysis.
Genomic DNA was isolated and treated with DpnI (NEB) for 90 min at 37 °C to restrict plasmid DNA. A 2.6-kb PCR product was generated using Platinum Taq HF (Invitrogen) and primers 5′-AATTTTGTAAATTTGTTTCATC-3′ and 5′-ACTTGCTTTGCCATTAACAGA-3′. 435-bp amplicons for sequencing by GS FLX++ chemistry (Eurofins Genomics), were generated with the primers 5′-ATCATGTGCCCCTTCTCTGT-3′ and 5′-CGTAGACTAGTGCTTTGATGACGCTTCTGTAT-3′ tagged with a unique 10 nucleotide multiplex-identifier (MID). Sequence alignments were performed using Clustal W42 (link) and MegAlign (Version 11.2.1. DNASTAR. Madison, Wisconsin).

Hollywood J.A., Lee C.M., Scallan M.F, & Harrison P.T. (2016). Analysis of gene repair tracts from Cas9/gRNA double-stranded breaks in the human CFTR gene. Scientific Reports, 6, 32230.

+ Open protocol

+ Expand

Maize Mutant Library Generation and Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

Generation of testcross populations, seedling growth, tissue pooling and deconvolution, and PCR screening were conducted as described in Studer et al. (2014) with the following modifications. A total of 450 Ac-positive kernels were planted per screen, and the tissue of 15 individual plants was pooled, for a total of 30 pools per screen. All PCR screens were conducted using Platinum Taq HF (Invitrogen) to facilitate long-distance screening of insertions distal to the target gene coding sequence. Individuals containing germinal insertions were backcrossed to T43, and single mutants were cross-pollinated to generate double mutants, which were validated by PCR genotyping (Supplemental Table S3).

Mertz R.A., Ellsworth P.Z., Ellsworth P.Z., Tausta S.L., Caemmerer S.v., Berg R.H., Nelson T., Carpita N.C., Brutnell T.P., & Cousins A.B. (2020). The maize Aliphatic Suberin Feruloyl Transferase genes affect leaf water movement but are dispensable for bundle sheath CO₂ concentration.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!