1200 series

Manufactured by Merck Group

The 1200 Series is a line of laboratory equipment manufactured by Merck Group. It is designed to provide reliable and precise performance for various laboratory applications. The core function of the 1200 Series is to facilitate the measurement, analysis, and processing of samples in a controlled and efficient manner. The specific details and intended uses of the 1200 Series may vary, and it is important to refer to the official product documentation for more comprehensive information.

Automatically generated - may contain errors

Lab products found in correlation

1100 series, by Merck Group (5 mentions) Chromolith rp 18e, by Merck Group (5 mentions) Diode array detector, by Agilent Technologies (3 mentions) 6130 series, by Merck Group (3 mentions) Inova 300, by Agilent Technologies (3 mentions)

7 protocols using 1200 series

Reverse Phase HPLC Analysis of Imazapyr and Phenol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Imazapyr and phenol concentrations were determined using reverse phase HPLC system equipped with a UV detector (Agilent 1200 series) with Supelco IL (250.0 × 4.6 mm, 5 µm separation column) and Supelco guard column (supelguard C18 (22.0 mm × 4.0 mm, 5 μm). The mobile phase was a mixture of 40% methanol and 60% water adjusted to pH 3.2 by adding formic acid, isocratic elution at a flow rate 1 mL·min⁻¹. The injection volume of 20 μL and 250 nm UV detection wavelength were used for Imazapyr. For phenol, 30 μL injection volume and UV detection wavelength of 270 nm was used.
Six different Imazapyr concentrations were used to build a calibration curves (R² value of 0.999). The limits of detection (LOD), and of quantitation (LOQ) were calculated, with values 9.82 ppb (LOD) and 29.47 ppb (LOQ) for phenol and 6.53 ppb (LOD) and 18.62 ppb (LOQ) for Imazapyr.

Bougarrani S., Sharma P.K., Hamilton J.W., Singh A., Canle M., El Azzouzi M, & Byrne J.A. (2020). Enhanced Photocatalytic Degradation of the Imidazolinone Herbicide Imazapyr upon UV/Vis Irradiation in the Presence of CaxMnOy-TiO2 Hetero-Nanostructures: Degradation Pathways and Reaction Intermediates. Nanomaterials, 10(5), 896.

+ Open protocol

+ Expand

Dansyl-Lipid II Transglycosylation Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipid II was prepared as described [32] . Dansyl fluorophore was attached to lipid II for spectroscopic detection (see structure and mass spectrum of dansyl-lipid II in Fig. S5) [33] . The mixtures were stored at -80 o C before HPLC analysis as described [33, 34] . 20 µL of samples were injected into an Agilent 1200 series equipped with a Supelco SAX1 anionexchange column and eluted using a gradient of NH4OAc/MeOH (20 mM to 500 mM). Half maximal inhibitory concentrations (IC50) of selected compounds were calculated by GraphPad Prism 7 with the model: Y=1/(1+10^((LogIC50-X)*h)) (Y, normalized response; X, log[inhibitor]; h, hillslope). The Dose-response curve for in vitro transglycosylation inhibition assays were shown in Fig. S2. The isatin-based compounds were measured in triplicate while vancomycin (positive control) was measured in duplicate, and half maximal inhibitory concentrations (IC50) were calculated as described [16] . HPLC chromatograms of in vitro transglycosylation assay of 5m were shown in Fig. S1.

Wang Y., Cheong W.L., Liang Z., So L.Y., Chan K.F., So P.K., Chen Y.W., Wong W.L, & Wong K.Y. (2020). Hydrophobic substituents on isatin derivatives enhance their inhibition against bacterial peptidoglycan glycosyltransferase activity. Bioorganic chemistry, 97.

+ Open protocol

+ Expand

Cytotoxicity of IQB-CBI Dimers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

Cytotoxicity of D801, D803, D05, D807, D809, D811, D813, D815 and D817 were tested against AML2 and HL60 cell lines. Results are shown in Table 1. Cell killing assay was performed after 3-day incubation with various IQB-CBI dimers.

TABLE 1

IC50

pg/mLD211D801D803D805D807D809D811D813D815D817

AML20.1916.214.652.21322860.130.280.070.13

HL600.4744.540.6142.655718500.290.720.120.19

General Methods:

¹H NMR spectra were recorded on a Varian Inova 300 or 500 MHz NMR instrument. Chromatographic purities were determined on an Agilent 1200 Series, 1100 Series or 6130 Series LC/MS system using a Merck Chromolith RP-18e analytical HPLC column (monolithic, 50×2 mm) and the following analytical HPLC method: injection volume 5 μL; flow rate 1 mL/min; 5→95% acetonitrile in water with 0.05% AcOH over 5 mins; Agilent diode array detector at λ=254, 220 or 195 nm; room temperature.

US10350218B2. Isoquinolidinobenzodiazepine (IQB)-1(chloromethyl)-2,3-dihydro-1H-benzo[e]indole (CBI) dimers (2019-07-16). Cellerant Therapeutics, Inc. [US]. Inventors: Jagath R. Junutula [US], Sean W. Smith [US], Dmitry Borkin [US], Sylvia Degrado [US], Sanjeevani Ghone [US].

+ Open protocol

+ Expand

NMR Spectroscopy and HPLC Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

General Methods:

+ Open protocol

+ Expand

Analytical HPLC Method for Chromatographic Purity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 29

[Figure (not displayed)]

Analytical methods: Chromatographic purities were determined on an Agilent 1200 Series, 1100 Series or 6130 Series LC/MS system using a Merck Chromolith RP-18e analytical HPLC column (monolithic, 50×2 mm) and the following analytical HPLC method: injection volume 5 μL; flow rate 1 mL/min; 5-95% acetonitrile in water with 0.05% AcOH (Method A) or 0.05% TFA (Method B) over 5 mins; Agilent diode array detector at 1=254, 220 or 195 nm; room temperature.

US11634508B2. Peptide conjugates of cytotoxins as therapeutics (2023-04-25). Cybrexa 2, Inc. [US]. Inventors: Daniel Richard Marshall [US], Johanna Marie Csengery [US], Robert John Maguire [US], Robert A. Volkmann [US].

+ Open protocol

+ Expand

NMR and HPLC Characterization Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 1

General Methods:

¹H NMR spectra were recorded on a Varian Inova 300 or 500 MHz NMR instrument. Chromatographic purities were determined on an Agilent 1200 Series or 1100 Series LC/MS system using a Merck Chromolith RP-18e analytical HPLC column (monolithic, 50×2 mm) and the following analytical HPLC method: injection volume 5 μL; flow rate 1 mL/min; 5→95% acetonitrile in water with 0.05% AcOH over 5 mins; Agilent diode array detector at λ=254, 220 or 195 nm; room temperature.

+ Open protocol

+ Expand

Synthesis of CLT-D202 Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 6

A synthesis scheme for CLT-D202 was performed as follows and is described in FIGS. 20A-D. Numbering is as in FIGS. 20A-D.

General Methods:

¹H NMR spectra were recorded on a Varian Inova 300 or 500 MHz NMR instrument. Chromatographic purities were determined on an Agilent 1200 Series or 1100 Series LC/MS system using a Merck Chromolith RP-18e analytical HPLC column (monolithic, 50×2 mm) and the following analytical HPLC method: injection volume 5 μL; flow rate 1 mL/min; 5→95% acetonitrile in water over 5 mins; Agilent diode array detector at λ=254, 220 or 195 nm; room temperature.

US09974864B2. Isoquinolidinobenzodiazepines (2018-05-22). Cellerant Therapeutics, Inc. [US]. Inventors: Jagath R. Junutula [US], Vasu Jammalamadaka [US].

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!