M3 plate reader

Manufactured by Molecular Devices

Sourced in United States

The M3 plate reader is a multi-mode microplate reader designed for a variety of assay applications. It provides accurate and reliable detection across multiple wavelengths and detection modes, including absorbance, fluorescence, and luminescence.

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Lab products found in correlation

Ethidium bromide, by Merck Group (2 mentions) P nitrophenyl phosphate substrate, by Merck Group (1 mentions) Abc ap, by Vector Laboratories (1 mentions)

Close spelling variants of this product

ASpectraMax M3 plate reader SoftMax M3 micro plate reader SpectraMax M3 plate reader

4 protocols using m3 plate reader

Ethidium Bromide Uptake Assay

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Bacterial cells were grown to mid-log phase (OD₆₀₀ of 0.4 to 0.5). Cells were harvested, washed, and resuspended in 0.1 M sodium phosphate buffer (pH 7) (previously incubated in the VAIN) to an OD₆₀₀ of 0.2. Cells were then incubated in the VAIN for 15 min at 37°C before a 100-μl aliquot was withdrawn to indicate time zero. Ethidium bromide (Sigma, UK) was added to a final concentration of 2 μg/ml, and fluorescence was measured at 530 nm excitation and 600 nm emission using a plate reader (Molecular Devices M3 plate reader, USA).

Abouelhadid S., North S.J., Hitchen P., Vohra P., Chintoan-Uta C., Stevens M., Dell A., Cuccui J, & Wren B.W. (2019). Quantitative Analyses Reveal Novel Roles for N-Glycosylation in a Major Enteric Bacterial Pathogen. mBio, 10(2), e00297-19.

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Colorimetric Nitrite Quantification in C. jejuni

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Determining the nitrite concentrations in the culture supernatants was done according to the method of Pittman et al. (25 (link)) with slight modifications. Briefly, an overnight culture of C. jejuni grown in brucella broth was diluted to an OD₆₀₀ of 0.1 in oxygen-limited condition. Samples were drawn every hour and centrifuged at 12,000 × g for 1 min. Supernatant was then diluted 1:5 with deionized water. Fifty microliters of diluted culture supernatant was mixed with 850 μl of 1% (wt/vol) sulfanilamide dissolved in 1 M HCl and 100 μl of 0.02% (wt/vol) naphthylethylenediamine. After 15 min, the absorbance at 540 nm was measured using a plate reader (Molecular Devices M3 plate reader, USA), and nitrite concentrations were determined by reference to a standard curve.

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CL-K1 Plasma Quantification by ELISA

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Plasma CL-K1 was assayed using a previously established sandwich ELISA method using two CL-K1 specific antibodies, with minor modifications [6 (link)]. Briefly, 384 well plates were coated with 20 μl of an anti-CL-K1 rabbit polyclonal antibody. After rinsing and blocking, 20 μl of diluted plasma samples and standard were incubated in duplicate. All plasma samples used in this study were thawed only once. After rinsing, the wells were incubated with a biotinylated anti-CL-K1 monoclonal antibody followed by ABC-AP (Vector Lab, Burlingame, CA) and then developed using p-nitrophenyl phosphate substrate (Sigma-Aldrich). Reactions were assayed for absorbance at 405 nm using an M3 plate reader (Molecular Devices).
From assays performed using plasma specimens from 27 healthy volunteers, the median of plasma CL-K1 levels was 245 ng/ml with interquartile range 160–273 ng/ml. The highest concentration measured in this group was 772 ng/ml. The CL-K1 reference range was found to be <619 ng/ml (normal donor mean + 2SD).
Plasma samples from a subpopulation of 216 patients (201 DIC and 15 non-DIC) were also assayed for plasma CRP, an acute phase protein, using ELISA (DuoSet, R&D Systems), according to the manufacturer’s instructions.

Takahashi K., Ohtani K., Larvie M., Moyo P., Chigweshe L., Van Cott E.M, & Wakamiya N. (2014). Elevated plasma CL-K1 level is associated with a risk of developing disseminated intravascular coagulation (DIC). Journal of thrombosis and thrombolysis, 38(3), 331-338.

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Ethidium Bromide Uptake Kinetics

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Bacterial cells were grown to mid-log phase (optical density at 600 nm [OD₆₀₀] = 0.4 to 0.5). Cells were harvested, washed, and resuspended in 0.1 M sodium phosphate buffer (pH 7; previously incubated in the VAIN) to an OD₆₀₀ of 0.2. The cells were then incubated in the VAIN apparatus for 15 min at 37°C before a 100-μl aliquot was withdrawn to indicate time zero. Ethidium bromide (Sigma, UK) was added to final concentration of 2 μg/ml, and fluorescence was measured at 530-nm excitation and 600-nm emission using an M3 plate reader (Molecular Devices).

Abouelhadid S., Raynes J., Bui T., Cuccui J, & Wren B.W. (2020). Characterization of Posttranslationally Modified Multidrug Efflux Pumps Reveals an Unexpected Link between Glycosylation and Antimicrobial Resistance. mBio, 11(6), e02604-20.

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