Thawed saliva was diluted 1:1 with phosphate buffered saline and prepared for flow cytometry (according to manufacturer's instructions) using the BD™ Cytometric Bead Array Human Inflammatory Cytokine Kit (BD Biosciences, Franklin Lakes, NJ, USA) to quantitatively measure IL‐8, IL‐1β, IL‐6, IL‐10, IL‐12p70, and tumor necrosis factor. Samples were acquired on an LSR II using FACSDiva™ Software v6.1.1 (BD Biosciences, Franklin Lakes, NJ, USA) and sample concentrations interpolated from standard curves for each cytokine using the radioimmunoassay option in GraphPad Prism v7.03.
Cytometric bead array human inflammatory cytokine kit
Manufactured by BD
Sourced in United States
The Cytometric Bead Array (CBA) Human Inflammatory Cytokine Kit is a multiplex assay designed to quantitatively measure the levels of multiple human inflammatory cytokines in a single sample. The kit utilizes flow cytometry technology to detect and analyze the concentrations of specific cytokines in cell culture supernatants, serum, plasma, or other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Human th1 th2 cytokine kit, by BD (2 mentions)
Fcap array software v3, by BD (2 mentions)
Fcap arraytm version, by BD (2 mentions)
Phorbol myristate acetate (pma), by InvivoGen (1 mentions)
Human serum, by Merck Group (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Cytometric bead array cba human inflammatory cytokine kit, by BD (1 mentions)
Cytoflex lx, by Beckman Coulter (1 mentions)
μtas wako i30, by Fujifilm (1 mentions)
Ec800 cell analyzer, by Sony (1 mentions)
Legendplex human inflammation panel 1 13 plex, by BioLegend (1 mentions)
Varioskan flash, by Thermo Fisher Scientific (1 mentions)
Ready set go, by Thermo Fisher Scientific (1 mentions)
Skanit, by Thermo Fisher Scientific (1 mentions)
Facscalibur, by BD (1 mentions)
Human il 6 high sensitivity elisa kit, by Thermo Fisher Scientific (1 mentions)
Human tnf ri tnfrsf1a quantikine elisa kit, by R&D Systems (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
Human chemokine kit, by BD (1 mentions)
Facsarray, by BD (1 mentions)
27 protocols using cytometric bead array human inflammatory cytokine kit
1
Quantifying Inflammatory Cytokines in Saliva
2
Neutrophil and Monocyte Response to G-CSF in NSG Mice
3
Investigating Cytokine Profiles in Immune Cell Lines
4
Quantifying Inflammatory Cytokines in PBC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BD Cytometric Bead Array (CBA) Human Inflammatory Cytokine Kit was used to quantitatively measure interleukin-8 (IL-8), interleukin-1β (IL-1β), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), and interleukin-12p70 (IL-12p70) protein levels in sera of PBC patients (see the characteristics of the patients in Table 2 ). All analyses were performed according to the manufacturer’s procedure (BD Cytometric Bead Array Human Inflammatory Cytokine Kit, #551811). Briefly, serum samples (25 μL) were diluted and incubated with beads conjugated with appropriate antibodies against the examined interleukin. Then, a detection reagent was added, which provided a fluorescent signal in proportion to the amount of the bound analyte. Thirty minutes later, the complexes (capture bead, analyte, and detection reagent) were measured using a CytoFLEX LX (Beckman Coulter, Brea, CA, USA) flow cytometer, and data were analyzed using CytExpert 2.0 data analysis software (Beckman Coulter, Brea, CA, USA).
5
Infliximab Therapy Biomarker Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected from patients just before the infliximab therapy after written informed consents were obtained. Serum samples were separated from the peripheral blood by centrifugation, and were stored at -30 °C for the assays. Procalcitonin concentrations were measured by μTAS Wako i30 (Wako Pure Chemical Industries, Osaka, Japan). The serum concentrations of IL-6, IL-8, and soluble tumor necrosis factor receptors (sTNFRs) were measured by flow cytometry using EC800 cell analyzer (Sony Corporation, Tokyo, Japan) with a BD Cytometric Bead Array Human inflammatory cytokine kit (BD Biosciences, San Jose, CA, USA) for IL-6 and IL-8, and Human Soluble TNFR Flex Set (Bio-Techne, Minneapolis, MN, USA) for sTNFR1 and 2. The detection limits were 3.0 pg/ml for IL-6, 3.6 pg/ml for IL-8, 5.2 pg/ml for sTNFR1, and 1.4 pg/ml for sTNFR2, respectively.
6
Multiplex Cytokine and Chemokine Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyse cytokine and chemokine levels in plasma and culture supernatants, the following kits were used: LEGENDplex Human Inflammation Panel 1 (13-plex) (Biolegend), Cytometric Bead Array Human Inflammatory Cytokine Kit (BD Biosciences) and Cytometric Bead Array Human Chemokine Kit (BD Biosciences).
7
Quantification of Inflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultured supernatants were collected and kept at -80°C until analysis. Concentrations of IL-1β, IL-6, IL-10, IL-12 and TNFα were determined simultaneously using Cytometric Bead Array (Human Inflammatory Cytokine Kit, BD Biosciences). Data acquisition was performed on a FACSCalibur (BD Biosciences) and analysis was performed using FCAP Array v3 (Soft Flow). IL-23 was quantitated by ELISA (Ready-SET-Go, eBioscience, San Diego, CA, USA) on a Varioskan Flash (Thermo Scientific) spectrophotometer and analyzed using the SkanIt™ (Thermo Scientific) program.
8
Cytokine Profiling in Inflammaging and Asthma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proinflammatory cytokines known to be involved in Inflammaging [4 (link)–8 ] (TNFα, IL-1β and IL-6, IL-8, IL-12 and sTNF RI) and to be important for the pathogenesis of bronchial asthma were selected for testing. The study also included IL-10, a cytokine with immunoregulatory and antagonistic properties. The serum levels of TNF-α, IL-1β and IL-6 were determined with the use of the commercial Thermo Fisher Scientific Ultrasensitive TNF alpha Human ELISA Kit, High Sensitivity IL-1 beta Human ELISA Kit and High Sensitivity IL-6 Human ELISA Kit. The serum level of soluble TNF RI was determined with the use of the commercial R&D Systems Human TNF RI/TNFRSF1A Quantikine® ELISA Kit. IL-12p70, IL-10 and IL-8 levels in the serum were measured with the BD™ Cytometric Bead Array Human Inflammatory Cytokine Kit. The samples were run on a BD™ LSR Fortessa flow cytometer and the results were analyzed with the BD™ FCAP Array software.
IL-10 was detectable only in 48/118 (40.7%), IL-12p70 in 20/118 (16.9%) and IL-1β in 10/118 (8.5%) of the study participants, and IL-10, IL-12p70 and IL-1β were not included in the analysis.
IL-10 was detectable only in 48/118 (40.7%), IL-12p70 in 20/118 (16.9%) and IL-1β in 10/118 (8.5%) of the study participants, and IL-10, IL-12p70 and IL-1β were not included in the analysis.
9
Serum Cytokine and Chemokine Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum concentrations of inflammatory cytokines (IL-8, IL-1β, IL-6, IL-10, TNFα and IL-12); Th1/Th2 cytokines (IL-2, IL-4, IL-5 and γ-interferon [IFNγ]); and chemokines (CCL2, CCL5, CXCL9, CXCL10) were measured in the residual serum from the etiology tests using the Cytometric Bead Array Human Inflammatory Cytokine Kit, Human Th1/Th2 Cytokine Kit and Human Chemokine Kit, respectively (BD Biosciences Pharmingen, San Diego, CA, USA). Flow cytometry (BD FACSArray) and the Software FCAP Array (BD Biosciences Pharmingen, San Diego, CA, USA) was used to perform the acquisition and the analysis, respectively. Lower limits of detection were: IL-8, 3.6 pg/ml; IL-1β, 7.2 pg/ml; IL-6, 2.5 pg/ml; IL-10, 3.3 pg/ml; TNFα, 3.7 pg/ml; IL-12, 1.9 pg/ml; IL-2, 2.6 pg/ml; IL-4, 2.6 pg/ml; IL-5, 2.4 pg/ml; IFNγ, 7.1 pg/ml; CCL5, 0.2 pg/ml; CXCL9, 2.5 pg/ml; CCL2, 2.7 pg/ml; CXCL10, 2.8 pg/ml. The maximum quantifiable level considered was supplied by the manufacturer: 10,000 pg/ml for all cytokines and chemokines. The assays were performed on two separate occasions by a technician blinded to etiological and clinical information. The residual serum samples from the etiology tests were kept frozen at -80 °C until cytokines and chemokines were measured. The same measurements and analyses were performed in the serum collected from healthy children (controls).
10
Measuring Inflammatory Cytokine Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The levels of inflammatory molecules IL6 and IL8 in culture supernatants were evaluated using the Cytometric Bead Array (CBA) Human Inflammatory Cytokine Kit (BD Biosciences) by flow cytometry following the manufacturer's instructions. Data were analyzed using FCAP Array software (Soft Flow Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!