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N n methylenebisacrylamide

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N,N'-methylenebisacrylamide is a chemical compound used as a cross-linking agent in various laboratory applications, such as gel electrophoresis and polyacrylamide gel preparation. It functions by forming covalent bonds between polymer chains, creating a stable and uniform gel matrix.

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Lab products found in correlation

Acrylamide, by Thermo Fisher Scientific (6 mentions) Ammonium persulfate, by Merck Group (5 mentions) Ammonium persulfate, by Thermo Fisher Scientific (4 mentions) Acrylic acid, by Thermo Fisher Scientific (4 mentions) N isopropylacrylamide, by Thermo Fisher Scientific (4 mentions) Acrylic acid, by Merck Group (3 mentions) Sodium borohydride, by Merck Group (3 mentions) Pvdf powder, by Polysciences (2 mentions) Ferrous chloride tetrahydrate, by Thermo Fisher Scientific (2 mentions) Methacrylic acid, by Thermo Fisher Scientific (2 mentions) N n n n tetramethylethylenediamine, by Merck Group (2 mentions) Chitosan, by Merck Group (2 mentions) Acrylamide, by Merck Group (2 mentions) Nitric acid, by Thermo Fisher Scientific (2 mentions) Potassium persulfate, by Thermo Fisher Scientific (2 mentions) Glycine, by Thermo Fisher Scientific (2 mentions) 2 acrylamido 2 methylpropanesulfonic acid, by Thermo Fisher Scientific (2 mentions) N n n n tetramethylethylenediamine, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

N,N’-methylenebisacrylamide (MBA, (4 citations) N,N′-Methylenebisacrylamide (MBA, C7H10N2O2), (2 citations)

29 protocols using n n methylenebisacrylamide

1

Synthesis and Characterization of PVDF Membranes

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The following chemicals were used as received: sodium borohydride (99.99%), acrylic acid (99%), PAA powder (MW = 2000) and palladium (II) acetate (98%), naphthalene_d8 (99 atom % D) were purchased from Sigma-Aldrich. Sodium hydroxide (1 M), sulfuric acid (0.5 M), hexanes and ferrous chloride tetrahydrate were obtained from Fisher Scientific. PVDF powder was purchased from Polysciences, Inc. Ammonium persulfate (98%), and N,N’-methylenebisacrylamide (NNMA, 99%) were received from Acros Organics. 3,3',4,4',5-pentachlorobiphenyl (> 97%, neat), biphenyl (> 97%, neat) and their analytical solution (100 ppm in hexane) were obtained from Ultra Scientific. Ethanol (200 proof) was bought from EMD Millipore Corporation. Sodium chloride was purchased from BDH. Full scale PVDF 700 microfiltration membranes were obtained from Nanostone Water, Inc. Deoxygenated water was obtained by purging N2 into deionized ultra-filtered (DIUF) water for 30 minutes.
Wan H., Briot N.J., Saad A., Ormsbee L, & Bhattacharyya D. (2017). Pore Functionalized PVDF Membranes with In-Situ Synthesized Metal Nanoparticles: Material Characterization, and Toxic Organic Degradation. Journal of membrane science, 530, 147-157.
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2

Membrane Fabrication and Characterization

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All chemicals used during the laboratory-scale membrane fabrication and other studies were reagent grade and used without further purification. 2,4,6-trichlorophenol (TCP, 98%), Acrylic acid (AA, 99%), ammonium persulfate (APS, >98%) and the enzyme laccase from Trametes versicolor (powder, light brown, ≥0.5 U/mg) were purchased from Sigma Aldrich. Poly(allylamine-hydrochloride) (PAH) and N, N′-methylenebisacrylamide (MBA, >99%) were obtained from Acros, New Jersey, NJ, USA. Bradford reagent was obtained from VWR.
The PVDF microfiltration membranes used for all the experiments were supplied from Nanostone/Sepro Membranes Inc., Oceanside, CA (PAA-PV400HA and PV700, pore diameter 200–450 nm, thickness of ~125 μm).
Sarma R., Islam M.S., Miller A.F, & Bhattacharyya D. (2017). Layer-by-Layer assembled laccase enzyme on stimuli-responsive membranes for chloroorganics degradation. ACS applied materials & interfaces, 9(17), 14858-14867.
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3

Synthesis of Stimuli-Responsive Polymeric Nanocarriers

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Methacrylic acid (MAA, 99%), 2,2′-Azobisisobutyronitrile (AIBN, 98%) and N,N′-methylenebisacrylamide (MBA, 96%) were purchased from Acros. N,N-dimetilaminoethylethacrylate (DMAEMA), Poly(ethyleneglycol)methylethermethacrylate (PEGMA; Mn = 475) and acetonitrile (ACN) were obtained from Sigma-Aldrich., N′-bis(acryloyl)cystamine (BAC, 98%) was purchased from Alfa Aesar. Daunorubicin HCl (DNR) was provided by Pharmacia & Upjohn.
Toniolo G., Efthimiadou E.K., Kordas G, & Chatgilialoglu C. (2018). Development of multi-layered and multi-sensitive polymeric nanocontainers for cancer therapy: in vitro evaluation. Scientific Reports, 8, 14704.
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4

Graphene Oxide-Based Dye Adsorption

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The monomers, itaconic acid (IA, 99%) and acrylamide (AM, 99%), catalyst, N,N,N′,N′-tetramethylethylenediamine (TEMED, 99%) and acetic acid (AA, 100%) were supplied from Merck. Chitosan (CS, medium viscosity) from crab shells with a degree of deacetylation of 80%, initiator, ammonium persulfate (APS, 98%) and basic dye, basic violet 14 (BV14, ≥80) were obtained from Sigma-Aldrich. N,N′-methylenebis (acrylamide) (MBA, 96%) was obtained from Acros Organics. Graphene oxide was obtained as explained in our previously reported procedure [29 ]. A stock solution of BV14 was prepared by dissolving BV14 in distilled water, and dye solutions of various concentrations were prepared by diluting this stock solution. HCl (0.1 M) and NaOH (0.1 M) were also obtained from Merck and used to adjust the medium pH. The chemical structure of BV14 dye is given in Figure 1.
TAMER Y, & BERBER H. (2022). Kinetic, isotherm, and thermodynamic studies for adsorptive removal of basic violet 14 from aqueous solution. Turkish Journal of Chemistry, 46(6), 2057-2071.
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5

Synthesis of Palladium Nanoparticles for Water Purification

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Acrylic acid (AA, 99%), potassium tetrachloropalladate (II) (K2PdCl4, 98%), sodium borohydride (NaBH4, 99.99%), 1, 2 dibromoethane (EDB, 99+ %) (Sigma-Aldrich, St. Louis, MO, USA), ammonium persulfate (APS, (NH4)2S2O8), dibutyl phthalate (DBP) (EM Science for Merck KGaA, Darmstadt, Germany). Ferrous chloride tetrahydrate (FeCl2·4H2O), trichloroethylene (TCE = 99.9+ %), sodium hydroxide (NaOH) solution, sodium chloride (NaCl), sulfuric acid (H2SO4) solution and nitric acid, pentane (Fisher Scientific, Fair Lawn, NJ, USA). Polyvinylidene fluoride polymer (PVDF) (Solef® 1015, MW = 516,000) (Solvay, France); polyvinylpyrrolidone (PVP) (MW=40,000) (Polysciences Inc., Warrington, PA, USA); Isopropanol (IPA, 99.9%), N, N′-methylenebisacrylamide (MBA > 99%) (Acros, New Jersey, NJ, USA). XPVDFSCO flat sheet microfiltration membranes (FS) with 0.40 μm pore size and 200 μm thickness (125 μm PVDF and 75 μm backing) (Nanostone, Oceanside, CA, USA). In all cases, HF and FS surface areas were based on the outer surface (10.9 cm2 on average) and the top surface area (17.3 cm2), respectively. All chemicals were of reagent grade and used as received.
Hernández S., Lei S., Rong W., Ormsbee L, & Bhattacharyya D. (2015). Functionalization of flat sheet and hollow fiber microfiltration membranes for water applications. ACS sustainable chemistry & engineering, 4(3), 907-918.
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6

Synthesis and Characterization of Polymer-Coated Nanoparticles

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The chemicals used in this study were purchased from the following companies: ethanol, formaldehyde, sodium hydroxide, ammonium persulfate (APS, 98%), ammonium hydroxide (30% NH3), nitric acid, and hydrochloric acid were all from EM Science, Hatfield, PA, USA, while potassium carbonate was from Aldrich, St. Louis, MO, USA. 3-(Trimethylsilyl)propylmethacrylate (MPS, 98.0%), N-isopropylacrylamide (NIPAM, 99%), acrylic acid (AA, 99.5%), and the cross-linker N,N′-methylenebisacrylamide (BIS, 96.0%) were obtained from Acros; tetraethylorthosilicate (TEOS), tetrakis(hydroxymethyl)phosphonium chloride (THPC), polyvinylpyrolidone (PVP, Mw ~55,000), and 3-aminopropyltrimethoxysilane (APTMS) were purchased from Aldrich, St. Louis, MO, USA; and, hydrogen tetrachloroaurate(III) hydrate came from Strem, Newburyport, MA, USA. Water was purified to a resistance of 18 MΩ (Academic Milli-Q Water System, Millipore Corporation, Burlington, MA, USA) and filtered using a 0.22 μm filter to remove any impurities. All glassware and equipment used in the experiment were first cleaned in an aqua regia solution (3:1, HCl:HNO3), then cleaned in a base bath (saturated KOH in isopropyl alcohol) and lastly, rinsed with Milli-Q water prior to use.
Kim B.S., Chen Y.T., Srinoi P., Marquez M.D, & Lee T.R. (2019). Hydrogel-Encapsulated Mesoporous Silica-Coated Gold Nanoshells for Smart Drug Delivery. International Journal of Molecular Sciences, 20(14), 3422.
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7

Synthesis and Characterization of Advanced Materials

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The following chemicals were used as received: sodium hydroxide (1 M), sulfuric acid (0.5 M), hexanes (> 99%), anhydrous sodium sulfate and ferrous sulfate were obtained from Fisher Scientific. Sodium borohydride (99.99%), sodium carboxymethyl cellulose (Mw~90k), potassium tetrachloropalladate (II) (98%), biphenyl_d10 (99 atom % D), trichloroethylene (99.5%) and tetrachloroethylene (> 99%) were purchased from Sigma-Aldrich. PVDF powder was purchased from Polysciences, Inc. Potassium persulfate (98%), N,N′-methylenebisacrylamide (NNMA, 99%) and methacrylic acid (99.5%, stabilized) were received from Acros Organics. Nitric Acid (AR select ACS) was ordered from Macron Fine Chemicals. 2-chlorobiphenyl (> 97%) and its analytical solution (100 ppm in hexane) as well as the analytical standards of chloroform (100 ppm in methanol), carbon tetrachloride (100 ppm in methanol) and hexachlorobutadiene (100 ppm in methanol) were obtained from Ultra Scientific. Ethanol (200 proof) was bought from EMD Millipore Corporation. Full scale PVDF 700 microfiltration membranes were obtained from Solecta, Inc. DVPP04700 membranes were purchased from Millipore. Deoxygenated water was obtained by purging N2 into deionized ultra-filtered (DIUF) water for 30 min.
Wan H., Islam M.S., Briot N.J., Schnobrich M., Pacholik L., Ormsbee L, & Bhattacharyya D. (2019). Pd/Fe nanoparticle integrated PMAA-PVDF membranes for chloro-organic remediation from synthetic and site groundwater. Journal of membrane science, 594, 117454.
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8

Stimuli-responsive Hydrogel Synthesis

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N-Isopropylacrylamide (NIPAAm, 97%), N-vinylpyrrolidone, and poly(ethylene glycol) diacrylate (PEG-DA, MW 575 g/mol) were purchased from Sigma-Aldrich (St. Louis, MO). N,N’-methylenebisacrylamide (BIS, 99%) was purchased from Acros Organics (Geel, Belgium). 2-Hydroxy-2-methyl-1-phenyl-1-propanone (Darocur 1173) and 1-[4-(2-Hydroxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one (Irgacure 2959) was purchased from Ciba Specialty Chemicals (Tarrytown, NY). Octamethylcyclotetrasiloxane (D4) and 1,3,5,7-tetra-methyl-1,3,5,7-tetra¬vinylcyclotetrasiloxane (D4Vi) came from Gelest, Inc. Dodecylbenzene-sulfonic acid (DBSA, BIO-SOFT® S-101) came from Stepan Co. (Northfield, IL). For hydrogel fabrication and other experiments, deionized water (DI) with a resistance of 18 MΩ·cm (Millipore, Billerica, MA) was used.
Abraham A.A., Means A.K., Clubb FJ J.r., Fei R., Locke A.K., Gacasan E.G., Coté G.L, & Grunlan M.A. (2018). Foreign Body Reaction to a Subcutaneously Implanted Self-Cleaning, Thermoresponsive Hydrogel Membrane for Glucose Biosensors. ACS biomaterials science & engineering, 4(12), 4104-4111.
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9

Cellulose Extraction from Water Hyacinth

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Cellulose was extracted from water hyacinth; acrylic acid and N,N-methylene–bis-acrylamide were obtained from ACROS Organics, Germany. Ammonium persulphate, acetone, toluene, ammonium hydroxide, hydrochloric acid, sulphuric acid, sodium hypochlorite, sodium chloride, calcium chloride and aluminium chloride were obtained from Loba Chemie, Mumbai, India.
Rop K., Mbui D., Njomo N., Karuku G.N., Michira I, & Ajayi R.F. (2019). Biodegradable water hyacinth cellulose-graft-poly(ammonium acrylate-co-acrylic acid) polymer hydrogel for potential agricultural application. Heliyon, 5(3), e01416.
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10

Hydrogel Formation with Catechol-Functionalized Monomers

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Acrylamide (AAm) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) were purchased from Sigma Aldrich (St. Louis, MO). N,N’-Methylene-bisAcrylamide (MBAA) and 2,2-dimethoxy-2-phenylacetophenone (DMPA) were obtained from Acros Organics (Geel, Belgium). Dimethyl sulfoxide (DMSO) was purchased from Fisher Scientific Co. (Pittsburgh, PA). Dopamine methAcrylamide (DMA), which contained the adhesive catechol and polymerizable methacrylate group, was synthesized as previously described.35 (link) Laponite XLG was a gift from Southern Clay Products, Inc. (Austin, TX).
Liu Y, & Lee B.P. (2016). Recovery property of double-network hydrogel containing mussel-inspired adhesive moiety and nano-silicate. Journal of materials chemistry. B, Materials for biology and medicine, 4(40), 6534-6540.
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