Comparison of groups for finding statistically significant differences was performed via two-way ANOVA’s in SigmaPlot (Version 11, SPSS Science, United States) at the significance level P < 0.05. In case of significant effects, the Holm–Sidak method was used for an all-pairwise multiple comparison. All other analysis of the data was done in Origin professional (versions 8.5 and 2015, OriginLab, United States).
Origin professional
Manufactured by OriginLab
Sourced in United States
Origin professional is a data analysis and graphing software developed by OriginLab. It provides tools for data processing, visualization, and presentation. The software offers a range of features for scientists, engineers, and researchers to analyze and interpret their data.
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Lab products found in correlation
4 protocols using origin professional
1
Statistical Analysis of Experimental Data
2
Statistical Analysis of Photosynthetic Parameters
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Comparison of groups for finding statistically significant differences compared to Zn treatment and virus infection was performed via the Mann–Whitney U test method in Origin professional (version 2015, Originlab, United States). This test was chosen considering the size of the samples and the fact that most of the photosynthetic parameters do not have normal distribution but become saturated.
3
Chlorophyll Fluorescence Analysis of X-ray Exposed Leaves
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Statistics was analysed in Origin Professional (versions 2015 and 2019; Originlab Corporation, Northampton, MA, USA). For analysis of chlorophyll fluorescence parameters that are saturating and do not have normal distribution (ΦPo, ΦRE1o, ΦEt2o and ΦPSII), the non-parametric Mann–Whitney U test [43 ] was used for comparison of the X-ray exposed and the adjacent leaf area (same size, bordering the exposed area). One-way repeated measures ANOVA and pair-wise comparison (Bonferroni test) were used to distinguish the effects of X-ray on the same leaf measuring area by comparing the values of NPQ_i1 before and after the X-ray exposure. This test deals with dependent variable which is subjected to repeated measurements—the independence assumption is not considered. The significance level of 0.05 was applied.
4
Photosynthesis and Respiration Monitoring
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Before the Cu treatments and after six weeks of Cu treatments, all the plants of one aquarium (1-3 plants) were placed into a 200 ml measuring chamber (custom made), maintained at 25 • C and oxygen exchange was measured by a WTW CellOx 325 oxygen electrode connected to an inoLab Oxi 740 termi-nal (Wissenschaftlich-Technische Werkstätten GmbH, Weilheim, Germany). Oxygen uptake in the dark and photosynthetic oxygen release was measured by exposing the plant to increasing irradiance. At the end, respiratory oxygen uptake in darkness was measured again. Data were recorded using the OxyCorder device with the software Oxywin 2.71 (Photon Systems Instruments, Brno, Czech Republic) and further data analysis was done in Origin Professional (versions 8.1-2015, Originlab, Northampton, USA).
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