Cell cycle progression was assessed by flow cytometry as reported previously.31 (link) Cardiac fibroblasts were collected and suspended in PBS. Fibroblast pellets were centrifuged at 1,000 g for 5 min and washed twice with PBS, resuspended in 70% ethanol, and fixed at 4°C for overnight. Fixed cells were then incubated with propidium iodide (50 μg/mL) and RNase A (100 μg/mL) for 30 min at room temperature, and DNA fluorescence was measured by ELITE Flow Cytometry (BD Biosciences) and CellQuest software (BD Biosciences).
Elite flow cytometry
Manufactured by BD
The ELITE Flow Cytometry is a laboratory instrument used for the analysis and sorting of cells and other particles based on their physical and chemical characteristics. It utilizes the principles of flow cytometry to provide rapid, accurate, and quantitative data on various cellular parameters.
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Lab products found in correlation
2 protocols using elite flow cytometry
1
Cell Cycle Analysis of Cardiac Fibroblasts
2
Quantifying Hippocampal Neuron Apoptosis
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The apoptosis rate of hippocampal neurons was carried out by flow cytometric analysis. Both attached and floating hippocampal neurons were harvested and washed twice with PBS, fixed in ice-cold 80% ethanol, and stored overnight at 4°C. Then, the cells were washed twice with PBS and 10 mg/mL RNase A was added. FITC-labeled Annexin V/PI staining was performed according to the manufacturer's instructions (Keygen, Nanjing, China). For each experiment, 20,000 cells were analyzed using Elite Flow Cytometry (BD Biosciences, San Jose, CA) and Cell Quest software (BD Biosciences). Triplicates were performed in all cases for the detection of early apoptotic cells.
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