Evaluations of device position after two weeks post-surgery were performed by MicroCT and MRI analysis. For MicroCT, see the procedures described previously. MRI was performed on a 9.4 T Bruker Biospec MRI system with a 30 cm bore, a 12 cm gradient insert, and an Autopac automated sample positioning system (Bruker Biospin, Billerica, MA). Respiratory signals were recorded using an MR-compatible physiologic monitoring system (SA Instruments, NY, USA). A warm water circulating system maintained body temperature. An actively decoupled, 4-channel phased array, receive-only radiofrequency coil designed specifically for the mouse brain (Bruker Biospin, MA, USA) was mounted on the bed. This assembly was centered inside a 72 mm quadrature volume coil in transmit-only mode (Bruker Biospin, Inc, MA, USA). Mice were imaged using an accelerated spin-echo sequence (Turbo Rapid Acquisition with Relaxation Enhancement, TurboRARE) oriented in axial, sagittal, and coronal directions. The following parameters were used: TR/TE = 1250 ms/21.3 ms, RARE factor 8, MTX = 256 x 256, FOV 3 x 3 cm, 7-13 slices of 0.75-1 mm thick (as needed for full brain coverage), flip back enabled, and 3 signal averages. The acquisition time was approximately 2 min per scan.
Autopac automated sample positioning system
Manufactured by Bruker
The Autopac automated sample positioning system is a laboratory equipment designed for efficient sample handling and positioning. It provides automated control and positioning of samples within the analysis environment.
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Lab products found in correlation
4 protocols using autopac automated sample positioning system
1
In-Vivo Imaging of Surgical Implants
2
High-Resolution MRI and microCT Imaging of Rat Brain
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MRI was performed on a 9.4T Bruker Biospec MRI system with a 30 cm bore, a 12 cm gradient insert, and an Autopac automated sample positioning system (Bruker Biospin Inc., Billerica, MA). An actively decoupled four-channel, phased array receive-only radiofrequency coil designed for rat brain (Bruker Biospin, Inc., Billerica, MA) was mounted on the bed. This assembly was centered inside a 72 mm quadrature volume coil in transmit-only mode (Bruker Biospin, Inc., Billerica, MA). Rats were imaged using an accelerated spin echo sequence (Rapid Acquisition with Relaxation Enhancement, RARE) oriented in axial, sagittal, and coronal directions. The following parameters were used: repetition time (TR)/echo time (TE) = 2000 ms/40 ms, RARE factor 8, acquisition matrix (MTX) = 256 × 256, field of view (FOV) 2 × 2 cm, 11–17 slices of 0.75–1 mm thick (as needed for full brain coverage), and 2 signal averages. Fat saturation was disabled, as this was found to slightly reduce image artifacts from the implanted devices. Acquisition time was ~2 min per scan. The reconstructed data were visualized in Amira 6.4 (FEI, Houston, TX). MRI and microCT images were manually registered as the image artifacts caused by the device precluded automatic image registration. A non-local means filter was applied to the CT data in Amira to reduce image artifacts.
3
Cardiac MRI Protocol for Atrial Imaging
4
In-Vivo Imaging of Surgical Implants
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Evaluations of device position after two weeks post-surgery were performed by MicroCT and MRI analysis. For MicroCT, see the procedures described previously. MRI was performed on a 9.4 T Bruker Biospec MRI system with a 30 cm bore, a 12 cm gradient insert, and an Autopac automated sample positioning system (Bruker Biospin, Billerica, MA). Respiratory signals were recorded using an MR-compatible physiologic monitoring system (SA Instruments, NY, USA). A warm water circulating system maintained body temperature. An actively decoupled, 4-channel phased array, receive-only radiofrequency coil designed specifically for the mouse brain (Bruker Biospin, MA, USA) was mounted on the bed. This assembly was centered inside a 72 mm quadrature volume coil in transmit-only mode (Bruker Biospin, Inc, MA, USA). Mice were imaged using an accelerated spin-echo sequence (Turbo Rapid Acquisition with Relaxation Enhancement, TurboRARE) oriented in axial, sagittal, and coronal directions. The following parameters were used: TR/TE = 1250 ms/21.3 ms, RARE factor 8, MTX = 256 x 256, FOV 3 x 3 cm, 7-13 slices of 0.75-1 mm thick (as needed for full brain coverage), flip back enabled, and 3 signal averages. The acquisition time was approximately 2 min per scan.
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