Hms ds50 slide stainer

Material solutions were prepared at a 2 mg/mL concentration, and the pH was adjusted to the desired level. All solutions were sterile filtered using a 0.2 μm syringe filter prior to use. Material solutions were introduced to the inlet of a microchannel by pipet. Solutions are drawn into the microchannel via capillary force to fill the entire channel within 1–3 s. After channel filling the excess material within the inlet is recovered from the inlet. Material in solution is allowed to adsorb for a predetermined amount of time (e.g., 5, 10, or 15 min), and then the channel is cleared either by vacuum or by pressurized air. The channel is then washed three times with a washing solution to remove material that is not well adsorbed to the channel surface. This process is then repeated with a complementary species for adsorption, and the entire process can be repeated to reach the desired number of layers of LbL film. After film assembly is complete the PDMS sheet can be removed from the substrate, leaving a microstrip of LbL film on the substrate. Dip LbL films were assembled using a Carl Zeiss HMS DS50 slide stainer. Films were built on either Si wafers or glass slides. Rinsing steps were performed using UltraPure DNase/RNase-Free water (Life Technologies) that was pH adjusted to match with the prior material adsorption step.