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Morgagni 268 tem

Manufactured by Ametek
Sourced in United States

The Morgagni 268 TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It provides advanced imaging capabilities with a high-resolution electron beam to enable detailed examination of samples at the nanoscale level.

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4 protocols using morgagni 268 tem

1

Multi-Modal Analysis of Bio-Samples

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LC-MS on Waters Acquity ultra Performance LC with Waters MICROMASS detector, rheological measurement on ARES-G2 rheometer; electron microscopy was performed on a FEI Morgagni 268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA); MTT assay for cell toxicity test on DTX880 Multimode Detector.
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2

HPLC Purification and Characterization of Biomolecules

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ALP was purchased from Biomatik, NBD-Cl from TCI and amino acids from GL
Biochem, PP1 from Sigma-Aldrich, PTP1B from Abcam. All the solvents and chemical reagents
were used directly as received from the commercial sources without further purification.
All the products were purified with Water Delta600 HPLC system, equipped with an XTerra
C18 RP column and an in-line diode array UV detector. Rheological data were obtained on TA
ARES G2 rheometer with 25 mm cone plate, confocal microscopy images on Leica TCS SP2
spectral confocal microscope. Electron microscopy imaging was performed on a FEI Morgagni
268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA, USA) or a 300 keV Tecnai F30
intermediate voltage TEM (FEI, Inc., Hillsboro, OR, USA) with a 4k CCD camera (GATAN).
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3

Comprehensive Multi-Analytical Characterization

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LC-MS
on Waters Acquity
ultra Performance LC with Waters MICROMASS detector, rheological measurement
on ARES-G2 rheometer; electron microscopy was performed on a FEI Morgagni
268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA); MTT assay
for cell toxicity test on DTX880 Multimode Detector.
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4

TEM Imaging of Cyanobacterial Cells

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For transmission electron microscopy (TEM) imaging of the cells, Synechococcus cultures grown in Fe(III)- and Si-supplemented A+ media were fixed in a solution of 2.5% gluteraldehyde + 2% Paraformaldehyde, dehydrated through an ascending ethanol series (50–100%), stained with 1% osmium tetroxide, embedded in Spurr low viscosity resin, thin sectioned (80–100 nm) using a Reichert-Jung Ultracut E microtome, and sections stained with 2% uranyl acetate. For images of the Fe(III)–Si precipitates, a drop of cell-free media was spread onto a cellulose film supported by a copper grid. TEM images were obtained using a Philips/FEI Morgagni 268 TEM operating at 80 kV equipped with a Gatan Orius Digital Camera using Gatan Digital Micrograph version 1.81.78 imaging software.
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