Uv 2100 spectrophotometer

Manufactured by Shimadzu

Sourced in Japan, United States

The Shimadzu UV-2100 spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light by a sample across a range of ultraviolet and visible wavelengths. It is used to analyze the chemical composition and properties of a wide variety of materials.

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37 protocols using uv 2100 spectrophotometer

Spectrophotometric Analysis of Myoglobin Redox

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A Shimadzu UV-2100 spectrophotometer (Shimadzu Scientific Instruments Inc., Columbia, MD, USA) was used to measure the myoglobin content at 525 nm. The myoglobin concentration was estimated using a millimolar extinction coefficient of 7.6 with a molecular weight of 16,110 [13 (link)] and measured in a g/100 g sample.
The absorbance (A) was also measured at 503, 525, 550, 557, 582, and 630 nm, and the proportions (%) of different redox stages of myoglobin were estimated using modified Krzywicki’s equations [17 (link)] as follows:

where R1 = A582/A525, R2 = A557/A525, and R3 = A503/A525.
The absorbances at 582, 525, 557, and 503 nm are designated as A582, A525, A557, and A503, respectively.

Panpipat W., Tongkam P., Çavdar H.K, & Chaijan M. (2023). Single Ultrasonic-Assisted Washing for Eco-Efficient Production of Mackerel (Auxis thazard) Surimi. Foods, 12(20), 3817.

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Spectrophotometric Assays for CAT and MPO

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The activities of CAT and MPO were determined both in extracellular and intracellular media. Both enzyme activities were determined with a Shimadzu UV-2100 spectrophotometer at 37 °C. MPO activity was measured by guaiacol oxidation [30 (link)]. The reaction mixture contained sodium phosphate buffer pH 7 and 13.5 mM guaiacol. The reaction was initiated by adding 300 mM H₂O₂, and changes at 470 nm were monitored. CAT activity was measured by the spectrophotometric method of Aebi [31 (link)] based on following the decomposition of H₂O₂ at 240 nm.

Annunziata G., Capó X., Quetglas-Llabrés M.M., Monserrat-Mesquida M., Tejada S., Tur J.A., Ciampaglia R., Guerra F., Maisto M., Tenore G.C., Novellino E, & Sureda A. (2021). Ex Vivo Study on the Antioxidant Activity of a Winemaking By-Product Polyphenolic Extract (Taurisolo®) on Human Neutrophils. Antioxidants, 10(7), 1009.

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Enzymatic Activity Profiling in PBMCs

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All the enzymatic determinations in PBMCs were determined using a Shimadzu UV-2100 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 37 °C. Catalase (CAT) activity was analysed by the spectrophotometric method of Aebi based on the decomposition of H₂O₂ [17 (link)]. Superoxide dismutase (SOD) activity was measured using an adaptation of the method of McCord and Fridovich [18 (link)]. Glutathione peroxidase (GPx) activity was determined using a modification of the spectrophotometric method of Glohé and Gunzler [19 (link)]. Glutathione reductase (GRd) activity was measured by means of an adaptation of the Goldberg and Spooner spectrophotometric method [20 ]. The activity of CAT was expressed as k(s⁻¹)/10⁹ cells and the other enzymatic activities as nkatal (nKat)/10⁹ cells.

Monserrat-Mesquida M., Quetglas-Llabrés M., Bouzas C., Capó X., Mateos D., Ugarriza L., Tur J.A, & Sureda A. (2021). Peripheral Blood Mononuclear Cells Oxidative Stress and Plasma Inflammatory Biomarkers in Adults with Normal Weight, Overweight and Obesity. Antioxidants, 10(5), 813.

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Neutrophil Enzyme Activity Assay

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The activities of CAT and MPO were determined both in the lysed neutrophil solution (intracellular media) and in the cell-free culture supernatant (extracellular media). Both enzyme activities were determined with a Shimadzu UV-2100 spectrophotometer at 37°C. MPO activity was measured by guaiacol oxidation [40 (link)]. The reaction mixture contained sodium phosphate buffer pH 7 and 13.5 mM guaiacol. The reaction was initiated by adding 300 mM H₂O₂, and changes at 470 nm were monitored. CAT activity was measured by the spectrophotometric method of Aebi [41 (link)] based on following the decomposition of H₂O₂ at 240 nm.

Capó X., Martorell M., Sureda A., Tur J.A, & Pons A. (2015). Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation. Oxidative Medicine and Cellular Longevity, 2015, 187849.

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Antioxidant Enzyme Activity Assays

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Catalase (CAT) activity was measured in plasma by the spectrophotometric method of Aebi [33 (link)]. Superoxide dismutase (SOD) activity was measured in plasma by an adaptation of the method of McCord and Fridovich [34 (link)]. All activities were determined with a Shimadzu UV-2100 spectrophotometer at 37 °C.

Capó X., Martorell M., Ferrer M.D., Sureda A., Pons V., Domingo J.C., Drobnic F., Martínez-Rodríguez A., Leyva-Vela B., Sarabia J.M., Herranz-López M., Roche E., Tur J.A, & Pons A. (2020). Calorie Restriction Improves Physical Performance and Modulates the Antioxidant and Inflammatory Responses to Acute Exercise. Nutrients, 12(4), 930.

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Antioxidant Enzyme Activity Assay

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Catalase (CAT) activity in plasma and PBMCs was measured by the spectrophotometric method of Aebi [38 ]. Superoxide dismutase (SOD) activity was measured in plasma and PBMCs by an adaptation of the method of McCord and Fridovich [39 ]. Glutathione reductase (GRd) activity was measured in PBMCs by a modification of the Goldberg and Spooner spectrophotometric method [40 ]. Glutathione peroxidase (GPx) activity was determined using an adaptation of the spectrophotometric method of Flohé and Gunzler [41 ]. All activities were estimated in PBMCs and/or plasma samples with a Shimadzu UV-2100 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 37 °C.

Busquets-Cortés C., Capó X., Bibiloni M.D., Martorell M., Ferrer M.D., Argelich E., Bouzas C., Carreres S., Tur J.A., Pons A, & Sureda A. (2018). Peripheral Blood Mononuclear Cells Antioxidant Adaptations to Regular Physical Activity in Elderly People. Nutrients, 10(10), 1555.

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Antioxidant and Prooxidant Enzyme Assays

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The plasma activities of the antioxidant enzymes—superoxide dismutase (SOD) and catalase (CAT)—and the prooxidant myeloperoxidase (MPO) were monitored in a Shimadzu UV-2100 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 37 °C. CAT activity was assessed following Aebi’s spectrophotometric technique, which relies on the breakdown of H₂O₂ at 240 nm [24 ]. A modification of McCord and Fridovich’s procedure was used to detect SOD activity at 550 nm [25 (link)]. MPO activity was lastly ascertained using guaiacol as a substrate by monitoring the development of polymerization products of oxidized guaiacol at 470 nm [26 (link)].

Quetglas-Llabrés M.M., Monserrat-Mesquida M., Bouzas C., Mateos D., Ugarriza L., Gómez C., Tur J.A, & Sureda A. (2023). Oxidative Stress and Inflammatory Biomarkers Are Related to High Intake of Ultra-Processed Food in Old Adults with Metabolic Syndrome. Antioxidants, 12(8), 1532.

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Measurement of Catalase and Superoxide Dismutase Activities

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The activities of catalase (CAT) and superoxide dismutase (SOD) were determined both in plasma as described elsewhere [34 (link),35 (link)]. Both enzyme activities were measured with a Shimadzu UV-2100 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 37 °C. Plasma CAT activity was measured using Aebi’s spectrophotometric method based on the decomposition of H₂O₂ [34 (link)]. Plasma SOD activity was measured by an adaptation of McCord and Fridovish’s method [35 (link)].

Monserrat-Mesquida M., Quetglas-Llabrés M., Abbate M., Montemayor S., Mascaró C.M., Casares M., Tejada S., Abete I., Zulet M.A., Tur J.A., Martínez J.A, & Sureda A. (2020). Oxidative Stress and Pro-Inflammatory Status in Patients with Non-Alcoholic Fatty Liver Disease. Antioxidants, 9(8), 759.

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Quantifying Antioxidant Enzyme Activities

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The activities of catalase (CAT) and superoxide dismutase (SOD) were determined in plasma and measured at 37 °C with a Shimadzu UV-2100 spectrophotometer (Shimadzu Corporation, Kyoto, Japan). CAT activity was measured using Aebi’s spectrophotometric method based on the decomposition of H₂O₂ at 240 nm [23 ] whereas SOD activity was determined by an adaptation of McCord and Fridovich’s method at 550 nm [24 (link)].

Quetglas-Llabrés M.M., Monserrat-Mesquida M., Bouzas C., Llompart I., Mateos D., Casares M., Ugarriza L., Martínez J.A., Tur J.A, & Sureda A. (2023). Mediterranean Diet Improves Plasma Biomarkers Related to Oxidative Stress and Inflammatory Process in Patients with Non-Alcoholic Fatty Liver Disease. Antioxidants, 12(4), 833.

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Antioxidant Enzyme Activities in Skeletal Muscle

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Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GRd) and glutathione peroxidase (GPx) activities were determined in gastrocnemius muscle homogenates. All activities were determined with a Shimadzu UV-2100 spectrophotometer at 37 °C. CAT activity was measured by the spectrophotometric method of Aebi based on the decomposition of H₂O₂ [30 (link)]. SOD activity was measured by an adaptation of the method of McCord and Fridovich [31 (link)]. GRd activity was measured by a modification of the Goldberg and Spooner spectrophotometric method [32 ]. GPx activity was measured using an adaptation of the spectrophotometric method of Flohe and Gunzler [33 (link)]. Results were normalized with protein concentration.

Annunziata G., Jimenez-García M., Tejada S., Moranta D., Arnone A., Ciampaglia R., Tenore G.C., Sureda A., Novellino E, & Capó X. (2020). Grape Polyphenols Ameliorate Muscle Decline Reducing Oxidative Stress and Oxidative Damage in Aged Rats. Nutrients, 12(5), 1280.

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