Quantum fx μct scanner

Manufactured by PerkinElmer

Sourced in United States

The Quantum FX μCT scanner is a compact, high-resolution micro-computed tomography (μCT) imaging system designed for non-destructive 3D visualization and analysis of small samples. It features a high-performance X-ray source and state-of-the-art detector technology to capture detailed, high-quality images of a variety of specimens.

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Lab products found in correlation

Quantum fx μct software, by PerkinElmer (1 mentions) Analyze 11, by PerkinElmer (1 mentions)

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Quantum FX (98 citations) Quantum FX μCT (4 citations)

6 protocols using quantum fx μct scanner

Micro-CT Analysis of Dental Mineralization

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The micro-computed tomography (micro-CT) analysis was performed with a Quantum FX μCT scanner (PerkinElmer, Waltham, MA, USA), allowing image resolution ranging from 10 to 295 μm using low doses of radiation. A 30 mm field of view was used, and the reconstruction was performed with NRecon software (Skyscan, Aartselaar, Belgium). The 3D images acquired were analyzed with Osirix DICOM viewer software (version 3.7.1) in multiplanar reconstruction mode.
A longitudinal follow-up was carried out, with measurements taken before and after the induction, after pulpotomy and at D4 or D15 (immediately before sacrifice). Representative images are shown in Figure 1 and Figure 4. Mineralization was assessed in 3D sections of several teeth. Roots were analyzed one by one with CTAn software (Skyscan).

Minic S., Florimond M., Sadoine J., Valot-Salengro A., Chaussain C., Renard E, & Boukpessi T. (2021). Evaluation of Pulp Repair after BiodentineTM Full Pulpotomy in a Rat Molar Model of Pulpitis. Biomedicines, 9(7), 784.

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Microarchitecture Analysis of MSC Pellets

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After in vivo implantation, pellets from two MSC donors (n = 2 donors, four pellets per donor and condition) were analyzed by the Quantum FX μCT scanner (PerkinElmer) using the following settings: isotropic voxel size of 10 μm, 70 kV, 160 mA, 5 mm field of view, 3 min scan (FINE setting) per sample. Raw μCT images were converted into 3D reconstructions using Quantum FX μCT software (PerkinElmer).

Narcisi R., Cleary M.A., Brama P.A., Hoogduijn M.J., Tüysüz N., ten Berge D, & van Osch G.J. (2015). Long-Term Expansion, Enhanced Chondrogenic Potential, and Suppression of Endochondral Ossification of Adult Human MSCs via WNT Signaling Modulation. Stem Cell Reports, 4(3), 459-472.

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Micro-CT Imaging of Rat Osteoarthritis

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All rats were imaged directly after CO₂/O₂ asphyxiation, using a Quantum FX μCT scanner (PerkinElmer, Waltham, MA) with parameters time = 3 min, isotropic voxel size = 30 μm³, tube voltage of 90 kV, and tube current = 180 μA. 3D reconstructed images were obtained, and serial 2D images were reconstructed using software Analyze 11.0 (PerkinElmer; RRID:SCR_009120). ImageJ software (ImageJ; RRID:SCR_003070) was used for all analyses. Serial 2D scans were evaluated for subchondral sclerosis, osteophytes, bone cysts, and loose bodies according to a multi‐modality scoring system evaluated previously (Panahifar et al., 2014). Joint alignment was evaluated using sagittal serial 2D scans. Periarticular dystrophic calcifications were quantified by the mean average of each pixel value above soft tissue pixel value of each μCT scan, based on histograms representing air, soft tissue, and calcified tissue values. To obtain comparable region of interests, the same distance from growth plate to the lower end of tibia on the scan was measured.

Rudnik‐Jansen I., Tellegen A.R., Pouran B., Schrijver K., Meij B.P., Emans P.J., de Gendt E., Thomas R.E., Kik M.J., de Visser H.M., Weinans H., Egas A., van Maarseveen E., Woike N., Mihov G., Thies J., Tryfonidou M.A, & Creemers L.B. (2019). Local controlled release of corticosteroids extends surgically induced joint instability by inhibiting tissue healing. British Journal of Pharmacology, 176(20), 4050-4064.

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Femur Lipid and Microarchitecture Analysis

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The femurs were collected after removing proximal muscle and connective tissue were and fixed in a 10% neutral buffered formalin solution overnight at 4 °C. For each mouse, one femur was used for bone microarchitecture analysis and lipid quantification using a Quantum FX μCT Scanner (PerkinElmer, Waltham, MA, USA), and the other femur was used to extract bone marrow for RNA analysis. For lipid determination, the femurs were decalcified for at least two weeks in a 14% EDTA solution, which was changed every three days. The bones were then stained in a 1% osmium tetroxide and 2.5% potassium dichromate solution at room temperature for 48 h and washed in tap water for at least 2 h. The bones were imaged by μCT. Bone mineral density (BMD) and lipid volume were quantified using the software Analyze 12.0 [21 (link)].

He Y., Zhang R., Yu L., Zahr T., Li X., Kim T.W, & Qiang L. (2023). PPARγ Acetylation in Adipocytes Exacerbates BAT Whitening and Worsens Age-Associated Metabolic Dysfunction. Cells, 12(10), 1424.

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Murine Lung Imaging Using μCT

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Mice were first anesthetized using 3–4% isoflurane and, subsequently, lung imaging was performed using a Quantum FX μCT scanner (PerkinElmer Inc.). Cardiac gating strategy was performed as previously described [14 ]. The images were acquired at 90 kV, 80 μA and the lungs were scanned 360° for 4 min to capture the entire lung. An image stack of 512 slices with voxel size of 0.04 mm × 0.04 mm × 0.04 mm was generated.

Birk G., Kästle M., Tilp C., Stierstorfer B, & Klee S. (2020). Automatization and improvement of μCT analysis for murine lung disease models using a deep learning approach. Respiratory Research, 21, 124.

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Femur Bone Micro-Architecture and Lipid Quantification

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Femurs were collected and fixed in 10% neutral buffered formalin overnight at 4°C, and subsequently used for bone micro-architecture analysis and lipid quantification. For micro-architecture analysis, a Quantum FX μCT Scanner (Perkin-Elmer) was used for scanning. For lipid quantification, a 14% EDTA solution was used to decalcify bone for at least 2 weeks with frequent changes. The bones were then stained for 48 hr in a 1% osmium tetroxide, 2.5% potassium dichromate solution at RT, washed in tap water for at least 2 hr, and imaged by μCT. The software Analyze 12.0 was used to quantify lipid volume and μCT scan parameters and performed according to their bone micro-architecture add-on. Marrow adipose sections were determined based on consistent 250 slice intervals measured from the identified growth plate of the femur.

Aaron N., Kraakman M.J., Zhou Q., Liu Q., Costa S., Yang J., Liu L., Yu L., Wang L., He Y., Fan L., Hirakawa H., Ding L., Lo J., Wang W., Zhao B., Guo E., Sun L., Rosen C.J, & Qiang L. (2021). Adipsin promotes bone marrow adiposity by priming mesenchymal stem cells. eLife, 10, e69209.

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