These studies were performed using the expression plasmid RUNX1-pCMV6-XL4 and empty pCMV6-XL4 vector (OriGene Technologies, Rockville, MD). This plasmid expresses RUNX1 variant 2 (NM__001001890), expressed from RUNX1 P2 promoter. HEL cells were transiently co-transfected using Lipofectamine 2000 (Invitrogen) with 100 ng – 650 ng of RUNX1-pCMV6-XL4 plasmid or empty vector and PCTP promoter luciferase reporter constructs (−1015/PGL3-basic). The cells were harvested 24–48 hours for luciferase activity, immunoblotting or RNA extraction.
Empty pcmv6 xl4 vector
Manufactured by OriGene
The empty pCMV6-XL4 vector is a plasmid that lacks any specific insert or gene. It is designed for cloning and expression purposes, providing a basic backbone for the insertion and expression of target genes in mammalian cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using empty pcmv6 xl4 vector
1
RUNX1-Dependent PCTP Promoter Activity
2
RUNX1 Transcription Factor Analysis
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