The hydrodynamic sizes of the particles in the colloidal samples and their stability were analyzed using dynamic light scattering (DLS) and ζ-potential measurements. The DLS measurements were performed using backscatter geometry with a 780 nm laser on a Nano-flex (Microtrac, Montgomeryville and York, PA, USA). For each sample, a set of five 2 min scans was collected. The ζ-potential data was collected using a Stabino (Particle Metrix GmbH, Ammersee, Germany).
Stabino
Manufactured by Particle Metrix
Sourced in Germany
The Stabino is a lab equipment product that measures the zeta potential of particles in liquid dispersions. It determines the surface charge characteristics of suspended particles. The Stabino provides information on the stability and interactions of colloidal systems.
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Lab products found in correlation
4 protocols using stabino
1
Colloidal Particle Size and Stability
2
Charge Density Determination of Polymer-Grafted Beads
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Beads (1 g) were dispersed in 100 mL Milli-Q H2O. The mixture was vigorously mixed with UltraTurrax (IKA) at 15 000 rpm until a homogeneous dispersion was obtained. Aliquots (1 mL) of the dispersions (pristine and in situ oxidized beads, respectively) were diluted with Milli-Q H2O to 100 mL and titrated with PDADMAC using a Stabino (ParticleMetrix) system. The charge density of PDADMAC is 0.307 μeq mL−1. The measurements were replicated three times for each sample and the surface charge densities were calculated according to eqn (1) : where V0 is the volume of titrant consumed to neutralize the sample, Vsample is the volume of the sample, and x is the concentration of the sample.
3
Zeta Potential Determination in Aqueous Dispersions
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A Stabino (Particle Metrix, Meerbusch, Germany) titration device was used for the determination of the ζ potential’s pH dependency and of the IEP. The particle concentration was set to 100 µg Fe/mL in an aqueous dispersion, and the pH was adjusted with 0.02 M HCl and 0.02 M NaOH, respectively. For the measurement, a 400-µm piston was used with a mixing time of 10 seconds and a titration step volume of 10 µL.
4
Nanoparticle Characterization by DLS and Zeta Potential
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The mean hydrodynamic diameter of the nanoparticles was determined via DLS using a Nanophox (Sympatec, Clausthal-Zellerfeld, Germany) cross-correlation spectrophotometer. pH-dependent ζ potential measurements were performed in aqueous solution with a Stabino® (Particle Metrix, Inning, Germany) in the range of pH 2 to 11 by the addition of 0.02 M HCl and 0.02 M NaOH, respectively. The experiments were performed in triplicates, and the results were averaged.
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