Drp cac

Experiments were carried out with a computer controlled PalmSens potentiostat. All electrochemical data were collected and analysed by the PalmSens PSTrace software. The electrochemical cell was purchased from Dropsens and consisted of a plexiglass flow cell and a disposable screen ink-printed platinum electrode (SPPtE, DRP-150, Dropsens, Italy). They consisted in a platinum disk-shaped (12.6 mm²) working electrode, a paste of silver/silver chloride pseudo-reference and a platinum strip counter electrode, on a ceramic substrate (3.3 cm × 1.0 cm). All the electrochemical measurements were referred to the screen-printed silver pseudo-reference electrode. The screen printed electrodes were interfaced to the potentiostat by a cable connector for SPEs (DRP-CAC, Dropsens, Italy). A Gilson MiniPuls 3 peristaltic pump and a Rheodyne low pressure injector with a 200-µL sample loop completed the apparatus.

Amperometric measurements were performed with a CHI812B potentiostat (CH Instruments, Austin, TX, USA) controlled by software CHI812B. Screen-printed carbon electrodes (SPCEs) (DRP-110, DropSens, Llanera (Asturias), Spain), consisting of a 4-mm diameter carbon working electrode, a carbon counter electrode and an Ag pseudo-reference electrode, were used as electrochemical transducers together with a specific cable connector (DRP-CAC, DropSens, S.L.), which acted as interface between the SPCEs and the potentiostat.
A Bunsen AGT-9 Vortex (Velp Scientifica, Usmate, MB, Italy) was employed for homogenization of the solutions. A Thermomixer MT100 constant temperature incubator shaker (Universal Labortechnik, Leipzig, Germany), a magnetic separator DynaMag-2 Magnet (ThermoFisher Scientific,Waltham, MA, USA)) and a Magellan V 7.1 (TECAN, Männedorf, Switzerland) ELISA plate reader were also employed. Once MBs were conveniently modified, they were captured onto the surface of the SPCEs and controlled by a neodymium magnet (AIMAN GZ) embedded in a homemade Teflon casing. All measurements were performed at room temperature.

Amperometric measurements were performed with a CH Instruments (Austin, TX, USA) model 812B potentiostat controlled by software CHI812B. Screen-printed carbon electrodes (SPCEs) (DRP-110, DropSens, Asturias, Spain), consisting of a 4 mm diameter carbon working electrode, a carbon counter electrode, and an Ag pseudo-reference electrode were used as electrochemical transducers. A specific cable connector (DRP-CAC, DropSens, S.L.) acted as interface between the SPCEs and the potentiostat. All measurements were performed at room temperature. A neodymium magnet (AIMAN GZ, Madrid, Spain) embedded in a homemade Teflon casing was used to magnetically capture, in a reproducible way, the modified-MBs on the surface of the SPCEs.
A Raypa steam sterilizer, a Telstar Biostar biological safety cabinet, an Optic Ivymen^® System incubator shaker (Comecta S.A, Sharlab, Madrid, Spain), a Bunsen AGT-9 Vortex for homogenization of the solutions, a DynaMag™-2 magnetic particle concentrator (123.21D, Invitrogen Dynal AS, Oslo, Norway), and a Sigma-Aldrich 1–15 K refrigerated microcentrifuge were also employed. The quality and quantity of the extracted RNA were evaluated by using a NanoDrop^® ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).