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Bactec peds plus bottles

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The BD BACTEC Peds Plus bottles are blood culture bottles designed for the detection of aerobic and anaerobic microorganisms in pediatric patients. The bottles facilitate the collection, transportation, and cultivation of blood samples to support the diagnosis of bloodstream infections.

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Lab products found in correlation

Bactec automated blood culture instrument, by BD (2 mentions) Bactec 9050, by BD (1 mentions) Dna blood mini kit, by Qiagen (1 mentions) Qiaamp dna blood mini kit, by Qiagen (1 mentions)

Spelling variants of this product

BACTEC bottles (10 citations) BACTEC Peds Plus (6 citations) Peds Plus (6 citations) BACTEC Peds Plus/F bottles (3 citations)

4 protocols using bactec peds plus bottles

1

Protocol for Detecting Bloodborne Pathogens

Cited 3 times
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For the Kilifi cohort and the Ugandan sites in the FEAST trial, blood samples for bacterial cultures were collected in BD BACTEC Peds Plus bottles and processed with a BD BACTEC automated blood culture instrument (Becton Dickinson) for initial detection of bacteria in the blood. BD BACTEC-positive samples were subcultured on standard media by routine microbiological techniques. Either biochemical test kits (API, bioMérieux), serological tests, or both were used to confirm suspected pathogens. Good Clinical Laboratory Practice was audited by Qualogy, and external quality assurance was provided by the UK National External Quality Assessment service. The following organisms were considered as contaminants: Bacillus species, coryneforms, Micrococcus species, coagulase-negative Staphylococcus, and citrobacter.
In all four studies, plasma PfHRP2 levels were quantitated using the previously published methodology (18 (link)). The lower limit of detection of the ELISA plasma assay is about 2 ng/ml. Patients in the Kilifi study and the FEAST trial were genotyped for the rs334 single-nucleotide polymorphism (HbS) using DNA extracted from fresh or frozen samples of whole blood as described in detail previously (25 (link), 39 (link)).
Watson J.A., Uyoga S., Wanjiku P., Makale J., Nyutu G.M., Mturi N., George E.C., Woodrow C.J., Day N.P., Bejon P., Opoka R.O., Dondorp A.M., John C.C., Maitland K., Williams T.N, & White N.J. (2022). Improving the diagnosis of severe malaria in African children using platelet counts and plasma PfHRP2 concentrations. Science translational medicine, 14(654), eabn5040.
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2

Protocol for Detecting Bloodborne Pathogens

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
For the Kilifi cohort and the Ugandan sites in the FEAST trial, blood samples for bacterial cultures were collected in BD BACTEC Peds Plus bottles and processed with a BD BACTEC automated blood culture instrument (Becton Dickinson) for initial detection of bacteria in the blood. BD BACTEC-positive samples were subcultured on standard media by routine microbiological techniques. Either biochemical test kits (API, bioMérieux), serological tests, or both were used to confirm suspected pathogens. Good Clinical Laboratory Practice was audited by Qualogy, and external quality assurance was provided by the UK National External Quality Assessment service. The following organisms were considered as contaminants: Bacillus species, coryneforms, Micrococcus species, coagulase-negative Staphylococcus, and citrobacter.
In all four studies, plasma PfHRP2 levels were quantitated using the previously published methodology (18 (link)). The lower limit of detection of the ELISA plasma assay is about 2 ng/ml. Patients in the Kilifi study and the FEAST trial were genotyped for the rs334 single-nucleotide polymorphism (HbS) using DNA extracted from fresh or frozen samples of whole blood as described in detail previously (25 (link), 39 (link)).
Watson J.A., Uyoga S., Wanjiku P., Makale J., Nyutu G.M., Mturi N., George E.C., Woodrow C.J., Day N.P., Bejon P., Opoka R.O., Dondorp A.M., John C.C., Maitland K., Williams T.N, & White N.J. (2022). Improving the diagnosis of severe malaria in African children using platelet counts and plasma PfHRP2 concentrations. Science translational medicine, 14(654), eabn5040.
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3

Malaria, Hemoglobin, and Bloodstream Infections

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Hematological, biochemical and malaria parasite data were derived by standard methods38 (link) while blood cultures were processed in BACTEC Peds Plus bottles using a BACTEC 9050 automated blood-culture instrument (Becton Dickinson, UK). Positive samples were sub-cultured on standard media by routine microbiological techniques31 (link). Quality assurance for all laboratory tests was provided by the UK National External Quality Assessment Service (www.ukneqas.org.uk). Within cases, we retrospectively tested for HbAS by PCR39 (link) using DNA extracted from fresh or frozen samples of whole blood using proprietary methods [ABI PRISM (Applied Biosystems, California, USA) or Qiagen DNA Blood Mini Kit (Qiagen, West Sussex, United Kingdom)]. Throughout the study, therefore, admitting clinicians were unaware of the HbS status of cases. For controls, we tested for HbAS, within 7 days of recruitment, using fresh blood samples collected into EDTA by alkaline electrophoresis on cellulose acetate gels (Helena TitanTMIII, Helena Biosciences, Gateshead, UK) using standard methods.
Uyoga S., Macharia A.W., Ndila C.M., Nyutu G., Shebe M., Awuondo K.O., Mturi N., Peshu N., Tsofa B., Scott J.A., Maitland K, & Williams T.N. (2019). The indirect health effects of malaria estimated from health advantages of the sickle cell trait. Nature Communications, 10, 856.
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4

Blood Sampling and DNA Extraction

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Blood samples (5 ml) were collected aseptically from the study children and immediately inoculated into BactecPeds Plus bottles (Becton Dickinson, Bactec system, Franklin Lakes, NJ) for microbiological culture. Total DNA was extracted from 200 μl of citrated-blood samples from the study children using QIAamp DNA blood Mini Kit (Qiagen, Hilden, Germany) following manufacturer’s instruction. The extracted DNA was re-suspended in 200 μl of elution buffer for use as template in PCR assays.
Das S., Ray U., Akhter I., Chattopadhyay A., Paul D.K, & Dutta S. (2016). Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis. BMC Microbiology, 16, 108.
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