Zfp36l2

The Western blot analysis was performed as described in a previous study [27 ]. The blotting bands were then incubated with primary antibodies overnight at 4 °C. The antibodies against the following proteins were used: Caspase-3 (Cell Signaling Technology #14220, 1:1000), PARP (Cell Signaling Technology #9542, 1:1000), BCL2 (Cell Signaling Technology #15071, 1:1000), PROCA1 (Biorbyt #orb1703, 1:1000), ZFP36L2 (Santa Cruz #sc-365908, 1:1000), c-Myc (Cell Signaling Technology #9402, 1:1000), phosphor-Akt (Cell Signaling Technology #4060, 1:1000), Akt (Cell Signaling Technology #4691, 1:1000), phosphor-PI3K (Cell Signaling Technology #17366, 1:1000), and PI3K (Cell Signaling Technology #4292, 1:1000). The internal reference antibodies were as follows: GAPDH (Cell Signaling Technology #5174, 1:1000) and β-tubulin (Proteintech #10068-1-AP, 1:1000). The secondary antibodies were as follows: HRP-conjugated Affinipure goat anti-rabbit immunoglobulin G (IgG) (H + L) (Proteintech #SA00001-2, 1:5000), and HRP-conjugated Affinipure goat anti-mouse IgG (H + L) (Proteintech #SA00001-1, 1:5000) were purchased from Proteintech (IL, USA). The images were captured by chemiluminescence imaging (Bio-Rad, CA, USA) and quantitated using the Quantity One system (Bio-Rad).