HepG2 and Huh-7 cell lines were purchased from JCRB Cell Bank. Huh-7 was maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai tesque), and HepG2 was maintained in DMEM with high glucose (Nacalai tesque). All culture media contained 10% fetal bovine serum (FBS; Gibco) and 50 units/50 μg/mL penicillin-streptomycin (Nacalai tesque).
Hepg2
Manufactured by Nacalai Tesque
Sourced in Japan
HepG2 is a cell line derived from human hepatocellular carcinoma. It is commonly used in cell-based in vitro assays and research applications involving liver-related studies.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Huh 7, by Nacalai Tesque (2 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (2 mentions)
Penicillin streptomycin, by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Nih 3t3, by Merck Group (1 mentions)
Hepg2, by Merck Group (1 mentions)
Tet system approved fbs, by Takara Bio (1 mentions)
Hek293, by Merck Group (1 mentions)
Nih3t3, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hek293, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
α mem, by Nacalai Tesque (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Plc prf 5, by Nacalai Tesque (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Endothelial cell basal medium 2, by Lonza (1 mentions)
3 protocols using hepg2
1
Cultivation of HepG2 and Huh-7 cell lines
2
Cell Line Maintenance and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human liver cancer line HepG2, human embryonic kidney cell line HEK293, human gastric cancer line AGS, and mouse fibroblast NIH3T3 were obtained from the Japanese Collection of Research Bioresources. Tetracycline‐inducible HepG2 cells were purchased from Takara in 2018. HepG2, HEK293, and NIH3T3 cells were maintained in DMEM (Sigma) supplemented with 10% FBS (Sigma). AGS cells were maintained in RPMI‐1640 (Sigma) supplemented with 10% FBS (Sigma). Tetracycline‐inducible HepG2 cells were maintained in α‐MEM (Nacalai Tesque) supplemented with 0.1 mM nonessential amino acids, 500 μg/ml G418, and 10% Tet system‐approved FBS (Takara).19 Cell lines were routinely monitored for Mycoplasma (4A Biotech Co.). Authentication of the cell lines was confirmed by short tandem repeating profiling every 6 months. The cells used for experiments were passaged within 10 times after thawing.
3
Cell culture protocol for HCC and HUVEC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four HCC cell lines and human umbilical vein endothelial cells (HUVECs) were used in this study: Hep-G2, HuH-7, and Li-7 cell lines (RIKEN Bioresource Center, Japan); PLC/PRF/5 cell line (JCRB Cell Bank, Japan); HUVECs (provided by T.K., Tokyo, Japan). HuH-7, Hep-G2, and PLC/PRF/5 cells were cultured in DMEM (Nacalai Tesque, Inc.,Japan). Li-7 cells were cultured in RPMI1640 (Nacalai Tesque) Both medium contained 10% heat-inactivated foetal bovine serum (Gibco, MA, USA), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Nacalai Tesque). HUVECs were cultured in Endothelial Cell Basal Medium-2 (Lonza, Switzerland) and Endothelial Cell Growth Medium-2 SingleQuots[™] Supplements and Growth Factors (Lonza) in collagen I-coated dishes (AGC TECHNO GLASS, Japan). All cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!