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Lncrna fish probe and fluorescent in situ hybridization kit

Manufactured by RiboBio
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Sourced in China

The LncRNA FISH Probe and Fluorescent in Situ Hybridization Kit is a laboratory tool designed for the detection and visualization of long non-coding RNA (lncRNA) molecules within cells. The kit provides the necessary reagents and probes to perform fluorescent in situ hybridization, a technique used to localize and quantify specific RNA sequences in cellular and tissue samples.

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Lab products found in correlation

Fv1000 confocal laser microscope, by Olympus (2 mentions)

Spelling variants of this product

FISH kit (288 citations) Fluorescent In Situ Hybridization Kit (275 citations) FISH probe (40 citations) LncRNA FISH Kit (12 citations) In situ hybridization kit (5 citations) FISH in situ hybridization kit (3 citations)

2 protocols using lncrna fish probe and fluorescent in situ hybridization kit

1

Cellular Localization of lncRNA RPL34-AS1

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To localize the cellular distribution of RPL34-AS1, the FISH assay was performed using the lncRNA FISH Probe and Fluorescent in Situ Hybridization Kit (Ribobio, China) according to the manufacturer’s guidelines. The probe cocktail included the 18S/cytoplasm probe, U6/nuclear probe, and lncRPL34-AS1 probe (DAPI and Cy3-labeled probes were synthesized for fluorescence signals). The results were captured by the OLYMPUS laser confocal microscope FV1000 (Olympus, Tokyo, Japan).
Zhang H., Pan E., Zhang Y., Zhao C., Liu Q., Pu Y, & Yin L. (2022). LncRNA RPL34-AS1 suppresses the proliferation, migration and invasion of esophageal squamous cell carcinoma via targeting miR-575/ACAA2 axis. BMC Cancer, 22, 1017.
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2

Localization of lncRPL34-AS1 by FISH

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To localize the cellular distribution of lncRPL34-AS1, the FISH assay was performed using the lncRNA FISH Probe and Fluorescent in Situ Hybridization Kit (Ribobio, China) according to the manufacturer's guidelines. The probe cocktail included the 18S/cytoplasm probe, U6/nuclear probe, and lncRPL34-AS1 probe (DAPI and Cy3-labeled probes were synthesized for uorescence signals). The results were captured by the OLYMPUS laser confocal microscope FV1000 (Olympus, Tokyo, Japan).
Zhang H., Pan E., Zhang Y., Zhao C., Liu Q., Lv Q., Zhou J., Liu R., Wang S., Pu Y., & Yin L. (2020). H3K4me3 and H3K27ac Activated lncRPL34-AS1 Acts as a Suppressor of Esophageal Squamous Cell Carcinoma via Targeting miR-575/ACAA2 Axis and Binding to ALOX12B and CAT.
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