Flow-cytometric analysis was performed to characterize myeloid dendritic cell (mDC) and monocyte (Mo) frequencies in PBMCs. All antibodies were purchased from BD Biosciences (San Jose, CA). Cells were stained according to BD protocols using the following mouse anti-human antibodies: CD3 (clone SP34-2), CD14 (clone M5E2), CD16 (clone 3G8), CD20 (clone 2H7), CD33 (clone P67.6), HLA-DR (clone G46.6), and CD11c (clone S-HCL-3). MDC frequencies were reported as percentage of mononuclear cells (MNC). Monocytes were further defined by gating as traditional monocytes (CD14++CD16−), inflammatory monocytes (CD14++CD16+) and patrolling monocytes (CD14dim CD16++) (see Additional file 1 : Figure S1). Samples were acquired on the LSR11 (BD; San Jose, CA) using FACS DIVA software and analysed with FlowJo (TreeStar, Inc., Ashland, OR).
Cd20 clone 2h7
Manufactured by BD
Sourced in United States
CD20 (clone 2H7) is a monoclonal antibody that binds to the CD20 antigen expressed on the surface of B cells. It is used in research applications to study B cell biology and development.
Automatically generated - may contain errors
Lab products found in correlation
Cd16 clone 3g8, by BD (1 mentions)
Cd3 clone sp34 2, by BD (1 mentions)
Cd14 clone m5e2, by BD (1 mentions)
Lsr11, by BD (1 mentions)
Facsdiva software, by Tree Star (1 mentions)
Cd33 clone p67.6, by BD (1 mentions)
Hla dr clone g46 6, by BD (1 mentions)
Milli q water, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions)
Lysing buffer, by BD (1 mentions)
Milli q plus 185 purification system, by Merck Group (1 mentions)
2 protocols using cd20 clone 2h7
1
Flow Cytometric Analysis of Myeloid Dendritic Cells and Monocytes
2
Multiparameter Blood Cell Phenotyping
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Materials. 8-Arm-poly(ethylene glycol) succinimidyl succinate of varying molecular weights Paraformaldehyde (PFA, 16% w/v) was purchased from Alfa Aesar. Lysing buffer was purchased from BD Biosciences. For human blood cell phenotyping, purified mouse anti-human CD3 (Clone OKT3), HLA-DR (Clone G46-6), CD45 (Clone HI30), CD56 (Clone NCAM16.2), CD66 (Clone B1.1/CD66), CD16 (Clone 3G8), CD11b (Clone ICRF44), CD19 (Clone HIB19), and CD20 (Clone 2H7) antibodies were purchased from BD Biosciences and purified anti-human CD14 (Clone M5E2) antibody was purchased from BioLegend (USA). Saline was obtained from Baxter Healthcare Pty. Ltd. (Australia). High-purity water (Milli-Q water) with a resistivity greater than 18.2 MΩ cm was obtained from a three-stage Millipore Milli-Q plus 185 purification system (Millipore Corporation, USA).
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