To evaluate the effect of itraconazole or lapatinib on cell proliferation, log phase OE33, FLO-1, KYSE70 and KYSE510 cells were plated in 6-well plates at 1×105 cells/well and cultured in cell culture medium supplemented with 2.5 μM itraconazole (Sigma), 2.5 μM lapatinib (Cayman Chemical) or DMSO for up to 6 days, with replacement of medium and drug every 24 hours. Every 48 hours, cell proliferation was measured by using a hemocytometer, and each experiment was performed in triplicate. The cell viability assay was performed using alamarBlue according to the manufacturer’s protocol (Thermo Fisher Scientific). OE33 and KYSE510 cells were plated in Corning 96-well black plates with clear bottom at 1×103 cells/well and cultured in cell culture medium supplemented with 2.5 μM itraconazole or DMSO for 72 hours. Fluorescence signal was measured at the designated timepoints under the fluorimeter (excitation at 544 nm and emission at 590 nm) using a POLARstar Omega Microplate Reader (BMG Labtech). Fluorescence signal was normalized to the cells treated with DMSO for 24 hours.
Itraconazole
Manufactured by Corning
Itraconazole is a laboratory product manufactured by Corning. It is a synthetic antifungal agent used in various research and testing applications. The core function of Itraconazole is to inhibit the growth and reproduction of fungi.
Automatically generated - may contain errors
2 protocols using itraconazole
1
Drug Effects on Cell Proliferation
2
Evaluating Anti-Proliferative Effects of Itraconazole and Lapatinib
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To evaluate the effect of itraconazole or lapatinib on cell proliferation, log-phase OE33, FLO-1, KYSE70, and KYSE510 cells were plated in 6-well plates at 1 × 105 cells/well and cultured in cell culture medium supplemented with 2.5 μmol/L itraconazole (Sigma), 2.5 μmol/L lapatinib (Cayman Chemical), or DMSO for up to 6 days, with replacement of medium and drug every 24 hours. Every 48 hours, cell proliferation was measured by using a hemocytometer, and each experiment was performed in triplicate. The cell viability assay was performed using AlamarBlue according to the manufacturer's protocol (Thermo Fisher Scientific). OE33 and KYSE510 cells were plated in Corning 96-well black plates with clear bottom at 1 × 103 cells/well and cultured in cell culture medium supplemented with 2.5 μmol/L itraconazole or DMSO for 72 hours. Fluorescence signal was measured at the designated timepoints under the fluorimeter (excitation at 544 nm and emission at 590 nm) using a POLARstar Omega Microplate Reader (BMG Labtech). Fluorescence signal was normalized to the cells treated with DMSO for 24 hours.
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