EpiLife culture medium with Ca2+ (60 μM), EpiLife defined growth supplement (EDGS), penicillin/streptomycin, and Lipofectamine 2000 reagent were purchased from Thermo Fisher Scientific (Waltham, MA USA). On-target plus siRNA was purchased from GE healthcare (Little Chalfont, UK). FNC coating mixture was purchased from Athena ES (Baltimore, MA, USA). Suramin hexasodium salt (an inhibitor of P2X and P2Y receptors), PPADS tetrasodium salt (an inhibitor of P2X and P2Y receptors), TNP-ATP triethylammonium salt (an inhibitor of P2X receptor), NF 157 (an inhibitor of P2Y11 and P2X1), ARL 67156 (an inhibitor of ecto-ATPases), and 10Panx (an inhibitor of Pannexin-1 channel) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Adenosine-5’-(γ-thio)-triphosphate tetralithium salt (ATPγS, a non-hydrolyzable ATP analogue), Brilliant blue G (BBG; an inhibitor of P2X receptors), and apyrase were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Epilife culture medium with ca2 60 μm
Manufactured by Thermo Fisher Scientific
Sourced in United States
EpiLife culture medium with Ca2+ (60 μM) is a proprietary, serum-free, and defined medium designed for the culture of epidermal keratinocytes and other epithelial cells. The medium contains 60 μM of calcium, which is an essential component for the growth and differentiation of these cell types.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (4 mentions)
10panx, by Bio-Techne (2 mentions)
Tnp atp triethylammonium salt, by Bio-Techne (2 mentions)
Apyrase, by Merck Group (2 mentions)
Brilliant blue g, by Merck Group (2 mentions)
Epilife defined growth supplement, by Thermo Fisher Scientific (2 mentions)
Suramin hexasodium salt, by Bio-Techne (2 mentions)
Ppads tetrasodium salt, by Bio-Techne (2 mentions)
Nf157, by Bio-Techne (2 mentions)
Arl67156, by Bio-Techne (2 mentions)
Adenosine 5 γ thio triphosphate tetralithium salt, by Merck Group (2 mentions)
On targetplus sirna, by GE Healthcare (2 mentions)
2 protocols using epilife culture medium with ca2 60 μm
1
Pharmacological inhibition of purinergic signaling
2
Pharmacological inhibition of purinergic signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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EpiLife culture medium with Ca2+ (60 μM), EpiLife defined growth supplement (EDGS), penicillin/streptomycin, and Lipofectamine 2000 reagent were purchased from Thermo Fisher Scientific (Waltham, MA USA). On-target plus siRNA was purchased from GE healthcare (Little Chalfont, UK). FNC coating mixture was purchased from Athena ES (Baltimore, MA, USA). Suramin hexasodium salt (an inhibitor of P2X and P2Y receptors), PPADS tetrasodium salt (an inhibitor of P2X and P2Y receptors), TNP-ATP triethylammonium salt (an inhibitor of P2X receptor), NF 157 (an inhibitor of P2Y11 and P2X1), ARL 67156 (an inhibitor of ecto-ATPases), and 10Panx (an inhibitor of Pannexin-1 channel) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Adenosine-5’-(γ-thio)-triphosphate tetralithium salt (ATPγS, a non-hydrolyzable ATP analogue), Brilliant blue G (BBG; an inhibitor of P2X receptors), and apyrase were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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