Pullulan was purchased from Meihua Group (Hebei, China). PAA (average MW of 450,000) was purchased from Aladdin Industrial Corporation Chemical (Shanghai). N-(3-Dimethylaminopropyl)-N’-Ethylcarbodiimide Hydrochloride (EDC) was purchased from Heowns Chemical (Tianjin, China). 4-Dimethylaminopyridine (DMAP) and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co., Ltd. Chlorin e6 (Ce6) was purchased from Frontier Scientific, Inc. aCD47 (Catalog no. BE0270) used in vivo was purchased from BioX Cell. AF790-labeled goat anti-mouse IgG (H&L) (Catalog no. 115-655-146) was purchased from Jackson ImmunoResear. Anti-CD3-PerCP-Cy5.5 (Catalog no. 551163), anti-CD4-APC (Catalog no. 553051), anti-CD8-FITC (Catalog no. 553030), anti-CD86-PE (Catalog no. 553692), anti-CD80-APC (Catalog no. 560016), anti-Foxp3-PE (Catalog no. 563101), anti-CD11c-FITC (Catalog no. 557400), anti-CD62L-BV421 (Catalog no. 562910), anti-CD44-APC (Catalog no. 559250) and anti-CD11b-PE-Cy7 (Catalog no. 552850) were purchased from BD Biosciences. Anti-mouse CD206-PE (Catalog no. 141705) and anti-mouse F4/80-FITC (Catalog no. 123107) were purchased from Biolegend. Human IgG ELISA Quantitation Set (Catalog no. E80-104) was purchased from Bethyl Laboratories, Inc.
4 dimethylaminopyridine dmap
Manufactured by Sinopharm
Sourced in China
4-dimethylaminopyridine (DMAP) is a heterocyclic organic compound that functions as a nucleophilic catalyst. It is commonly used in organic chemistry laboratory settings to facilitate various chemical reactions.
Automatically generated - may contain errors
Lab products found in correlation
Dspe peg2000 mal, by NOF corporation (3 mentions)
Dicyclohexylcarbodiimide dcc, by Sinopharm (2 mentions)
Anti cd44 apc, by BD (1 mentions)
Anti cd4 apc, by BD (1 mentions)
Anti cd8 fitc, by BD (1 mentions)
Anti cd11b pe cy7, by BD (1 mentions)
Human igg elisa quantitation set, by Fortis Life Sciences (1 mentions)
Anti cd62l bv421, by BD (1 mentions)
Pe anti mouse cd206, by BioLegend (1 mentions)
Anti foxp3 pe, by BD (1 mentions)
Anti cd11c fitc, by BD (1 mentions)
Fitc anti mouse f4 80, by BioLegend (1 mentions)
Anti cd80 apc, by BD (1 mentions)
Anti cd86 pe, by BD (1 mentions)
Chlorin e6 ce6, by Frontier Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (1 mentions)
Anti cd3 percp cy5, by BD (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Hoechst 33324, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent, by Merck Group (1 mentions)
5 protocols using 4 dimethylaminopyridine dmap
1
Pullulan-based Immunotherapeutic Delivery
2
Multifunctional Theranostic Nanoplatform
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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000) and hydrogenated soybean phosphatidylcholine (HSPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Doxorubicin hydrochloride (DOX·HCl) was supplied by Hisun Pharmaceutical (Zhejiang, China). Cholesterol was obtained from Hushi Chemical (Shanghai, China). ICG was supplied by TCI (Tokyo, Japan). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT reagent) and Hoechst 33,324 were purchased from Sigma-Aldrich (St Louis, MO, USA). Triethylamine (TEA), NaN3, and 4-dimethylaminopyridine (DMAP) were obtained from Sinopharm Chemical Reagent (Shanghai, China). Live/Dead viability/cytotoxicity kit was purchased from Invitrogen (Carlsbad, CA, USA). 1,3-Diphenylisobenzofuran (DPBF) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). The chemicals and solvents were of analytical grade and used as received without further purification.
3
Preparation of Functionalized Graphene Oxide
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Tryptone and yeast extract were purchased from Suzhou Biogene Biotechnology Co.,Ltd. Graphite powder, sulfuric acid (H2SO4), hydrochloric acid (HCl), hydrogen peroxide (H2O2), dimethylacetamide (DMAC), triethylamine (TEA), methacryloyl chloride (MAC), azobisisobutyronitrile (AIBN), methacrylic acid (MAA), butyl methacrylate (BMA), N-hydroxy succinimide (NHS), N,N’-Dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), cyclohexanone, petroleum ether, ether and inorganic salts were all in analytical reagent grade were commercially obtained from Sinopharm Chemical Reagent Co.,Ltd. and used as received without further treatment. HPLC grade methanol was purchased from Shanghai Qiangshun Chemical Reagent Co.,Ltd. HPLC grade DMF was obtained from Tedia Company Inc.
4
Peptide-Functionalized Paclitaxel-Loaded Liposomes
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Distearoyl-glycerophosphoethanolamine-polyethyleneglycol2000-maleimide (DSPE-PEG2000-Mal) was purchased from NOF Corporation (Tokyo, Japan). Peptide (CYKLEGTTRLTRKRGLKLA) was custom synthesized by GL Biochem (Shanghai, China). Paclitaxel (PTX) was purchased from Norzer Pharmaceutical Co., Ltd. Alpha linolenic acid (ALA) was purchased from ANPEL Laboratory Technologies (Shanghai, China). Soybean phosphatidylcholine (SPC), unesterified cholesterol (UC), triolein (TO) and cholesteryl oleate (CO) were from Lipoid (Ludwigshafen, Germany), Wako Ltd. (Tokyo, Japan), Sinopharm Chemical Reagent Co., Ltd (Beijing, China) and InnoChem Science & Technology Co., Ltd. (Beijing, China), respectively. Solutol® HS 15 was produced by BASF (Germany). Dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were obtained from Sinopharm Group Co., Ltd. Cell Counting Kit-8 (CCK- 8) was obtained from SolarbioScience & Technology Co., Ltd (Beijing, China). Coumarin-6 (COU) was purchased from Sigma-Aldrich Company (USA). TUNEL Kit was obtained from KeyGEN Biotech Co., Ltd. (Nanjing, China). Enzyme-linked Immunosorbent Assay (ELISA) Kit for LDLR was obtained from USCN Life Science Inc. Modified eagle medium (MEM), DMEM, penicillin-streptomycin, trypsin and Hoechst 33258 were obtained from Macgene Technology (Beijing, China).
5
Synthesis of Pyrene-Labeled Cyclodextrin Polymers
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N,N-dimethylacrylamide (DMAA, 99%), α-cyclodextrins (α-CD, 98%), β-cyclodextrins (β-CD, 98%), γ-cyclodextrins (γ-CD, 98%) and 1-pyrenebutyric acid (PyBA, 97%) were purchased from J&K Scientific Co., Ltd. (Shanghai, China). Azobisisobutyronitrile (AIBN) was purchased from Tianjin Bodi Chemical Co., Ltd. (Tianjin, China) and purified by recrystallization from ethanol. 2-Hydroxyethyl methacrylate (HEMA, 99%) was purchased from Shanghai Aladdin Chemical Reagent Co., Ltd. (Shanghai, China). Dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Tetrahydrofuran (THF), dichloromethane, diethyl ether and ethanol were used as received from commercial sources. All other reagents were used without further purification.
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