BCAA concentrations in plasma and follicular fluids were determined by the liquid chromatography-tandem mass spectrometric method using a 3200 Q TRAP triple-quadrupole linear ion trap mass spectrometer (Applied Biosystems, Darmstadt, Germany) and a high-performance liquid chromatography system (Dionex, Sunnyvale, CA, USA).33 (link)
3200 q trap triple quadrupole linear ion trap mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The 3200 Q TRAP triple-quadrupole linear ion trap mass spectrometer is a highly sensitive and versatile analytical instrument used for the identification and quantification of a wide range of compounds in complex samples. It combines the capabilities of a triple-quadrupole mass spectrometer with a linear ion trap, enabling advanced mass analysis techniques.
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Lab products found in correlation
2 protocols using 3200 q trap triple quadrupole linear ion trap mass spectrometer
1
BCAA Quantification in Biological Fluids
2
Fasting Hormone and Metabolite Evaluation in IVF
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Fasting blood samples from all subjects were collected on days 2–5 of a natural cycle or when amenorrhea for over 40 days with follicle diameter not exceeding 10 mm for basal FSH, luteinizing hormone (LH), androstenedione (A), and E2 assay. Fasting glucose and insulin levels were measured within 2 h after blood sampling on the day of oocyte retrieval. FF was aspirated from the leading follicle from each ovary. Only FF macroscopically free from blood was retained for further determinations. FF samples were centrifuged for 10 min at 3000 g and then stored at -80°C until use.
AA concentrations were determined by the liquid chromatography-tandem mass spectrometric method using a 3200 Q TRAP triple-quadrupole linear ion trap mass spectrometer (Applied Biosystems, Darmstadt, Germany) and a high performance liquid chromatography system (Dionex, Sunnyvale, CA, USA) as described previously [13 (link)]. Sex hormones were determined by chemiluminescence (Siemens, Germany).
AA concentrations were determined by the liquid chromatography-tandem mass spectrometric method using a 3200 Q TRAP triple-quadrupole linear ion trap mass spectrometer (Applied Biosystems, Darmstadt, Germany) and a high performance liquid chromatography system (Dionex, Sunnyvale, CA, USA) as described previously [13 (link)]. Sex hormones were determined by chemiluminescence (Siemens, Germany).
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