Nh4cooh

Manufactured by Merck Group

Sourced in United States, Italy

NH4COOH is a chemical compound that is commonly used as a laboratory reagent. It serves as a source of ammonium ions (NH4+) and bicarbonate ions (HCO3-) in various chemical experiments and analyses. The compound is a white, crystalline solid that is soluble in water.

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Ammonium formate (NH4COOH) (11 citations)

3 protocols using nh4cooh

Peptide Fractionation by Strong Cation Exchange

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Dried peptides were resuspended in 100 µl buffer SCX-A, consisting of 10 mM NH₄COOH (Sigma-Aldrich) in 20% acetonitrile (Thermo Fisher Scientific), pH 2.7, vortexed, sonicated for 5 min and spun at 15,000 g for 1 min. The supernatant was loaded on a 2.1×100 mm polysulfoethyl A column with 5 µm, 200 Å particles (PolyLC Inc.) and eluted with buffer SCX-B (500 mM NH₄COOH in 20% acetonitrile, pH 2.7) using a gradient of 0-10% buffer SCX-B for 2.5 min, 10-50% buffer SCX-B for 20 min, 50-100% buffer SCX-B for 7.5 min and 100% buffer SCX-B for 10 min. Twelve fractions were manually collected over 39 min, with fraction 1 collected from 0 to 12 min and fraction 12 from 32 to 39 min. The remaining ten fractions were collected at 2-min intervals between 12 min and 32 min. All SCX fractions were dried in a SpeedVac concentrator.

Krasny L., Bland P., Burns J., Lima N.C., Harrison P.T., Pacini L., Elms M.L., Ning J., Martinez V.G., Yu Y.R., Acton S.E., Ho P.C., Calvo F., Swain A., Howard B.A., Natrajan R.C, & Huang P.H. (2020). A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts. Disease Models & Mechanisms, 13(7), dmm044586.

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Synthesis and Characterization of Ionic Liquids

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Two neutral compounds (BOH and THBP), HPLC-grade acetonitrile (ACN), ammonium formate (99%, NH₄COOH), formic acid (95%), acetic acid (99%), dimethyl sulfoxide (99%, DMSO) and TM-β-CD (90%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The other two neutral compounds (TFAE and TSO) were purchased from TCI AMERICA (Tokyo, Japan). Sodium acetate (NaOAc), sodium hydroxide (NaOH) (50% w/w), sodium phosphate dibasic heptahydrate and sodium phosphate monobasic monohydrate were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Triply deionized (DI) water used in this experiment was obtained from Barnstead Nanopure II water system (Barnstead International, Dubuque, IA, USA).
The reagents ω-undecylenyl alcohol, 2-bromoethylamine hydrobromide, pyridine anhydrous, L-leucinol, L-alaninol, L-valinol, L-isoleucinol, (1S,2R)-ephedrine hydrochloride, formic acid (≥95), formaldehyde (37% (wt/wt) solution in water) used for the synthesis of ILs were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Triphosgene was obtained from TCI AMERICA (Tokyo, Japan). Sodium sulfate anhydrous and sodium bicarbonate were purchased from Fisher Scientific (Fair Lawn, NJ, USA).

Liu Y, & Shamsi S.A. (2014). Combined use of chiral ionic liquid surfactants and neutral cyclodextrins: Evaluation of ionic liquid head groups for enantioseparation of neutral compounds in capillary electrophoresis. Journal of chromatography. A, 1360, 296-304.

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Lipidomic Analysis of Biological Samples

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ULC-MS grade acetonitrile, ethanol, 2-propanol, methanol and ultra-pure water were purchased from Biosolve (Chimie SARL, Dieuze, France; BV, Valkenswaard, Netherlands). Chloroform was purchased from Carlo Erba (Milan, Italy). NH₄COOH, NH₄F, and butylated hydroxytoluene (BHT) were purchased from Sigma Aldrich (Milan, Italy). The Q-TOF calibration solution was prepared in acetonitrile from Agilent Technologies Tuning mix (HP0321 solution, Agilent Technologies, Santa Clara, USA).
N-palmitoyl-d31-D-erythro-sphingosine (d31-Cer[NS]34:1, MW 569.1), N-[26-oleoyloxy(d9) hexacosanoyl]-d-erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1(d9), MW 967.6) and N-[26-oleoyloxy hexacosanoyl]-d-erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1), MW 958.6) were purchased from Avanti Polar Lipids (Alabaster, USA). Deuterated cholesterol sulfate sodium salt (d7-CHS, MW 495.3) and Hexadecanoic-9,9,10,10,11,11,12,12,13,13,14,14,15,15,16,16,16-d17 acid (d17-PA, MW 273.5) were purchased from CDN Isotopes Inc., (Pointe-Claire, Canada). Stock solution of the internal standard (iSTD) mixture of deuterated compounds was prepared in 2-propanol with the following concentrations: d7-CHS 40 µM, d17-PA acid 80 µM, d31-Cer16:0 10 µM. d9-CER[EOS] and CER[EOS] mixture (2 µM each in Chloroform/methanol (2:1)) was introduced to confirm identification of O-acylceramides.

Maiellaro M., Bottillo G., Cavallo A, & Camera E. (2024). Comparison between ammonium formate and ammonium fluoride in the analysis of stratum corneum lipids by reversed phase chromatography coupled with high resolution mass spectrometry. Scientific Reports, 14, 40.

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