Opmi 1 fc

All of the animals underwent general anesthesia by intraperitoneal injection of ketamine and xylazine. Using a surgical microscope (Zeiss OPMI 1FC), laminectomy of one vertebra at thoracic level T8–T9 was performed. We used a dorsal hemi-crush model as previously described by Chen et al., (2017) . A pair of no. 5 Dumont forceps (Fine Science Tools) was then used to compress the dorsal spinal cord at a depth of 0.7 mm across the entire width of the cord for 5 s. This dorsal hemi-crush model allowed for better survival following surgery as compared with full spinal cord complete crush and thus was used throughout this study, especially given that mice overexpressing astrocyte-specific LZK exhibited reduced survival even without injury.

All MRM was carried out on a 600 MHz (14.1 T) vertical-bore magnet (Oxford Instruments) interfaced to a Bruker Biospin console. Imaging gradient strengths up to 66 T/m were provided by a newly designed and fast-switching (1.1 T/m/A) planar gradient system (Bruker Biospin, Z110828, B6406). Initially, myofibers were embedded in low-melting point agarose (22-110-617, Fisher) and positioned by hand with the aid of a dissecting scope (Zeiss, OPMI 1-FC). Next, an agarose block containing embedded samples was placed directly on the RF microcoil. Lastly, the tissue well was sealed using PCR film (ABgene, AB-0558) to prevent leakage and ensure the physical stability of samples. Imaging protocols were repeated for each stain concentration and incubation time tested (n = 3).
In MRM experiments targeting myonuclei, micro surface-coils of 200 μm (inner diameter) (Bruker Biospin, B6371) and 500 μm (inner diameter) (Bruker Biospin, B6370) were utilized. Three-dimensional FLASH datasets containing the nucleus and neighboring intracellular regions of single muscle fibers were segmented, analyzed, and reconstructed using 3D image analysis software (Amira 5.4.0; Visage Imaging) in order to visualize subcellular structures from multiple angles.

The DSN and OOr attached to the fMCs of the major alar cartilage were investigated in 76 samples from 38 embalmed Korean adult cadavers (20 males, 18 females; mean age 70 years, age range 33–93 years). The muscle fibers attached to the nose in 34 of 38 cadavers were dissected. The noses of all samples were removed en bloc with the septal nasal cartilage and the muscles attached to the facial bones. The inner surfaces of the removed noses and their attached muscles were carefully dissected under a surgical microscope (OPMI 1FC, Carl Zeiss, Oberkochen, Germany). The muscle fibers attached to the fMCs were revealed by cutting the inferior part of the septal nasal cartilage. When the DSN and OOr were observed with crossing fibers attached to the fMCs or nasal septum, these fibers were followed to identify their arrangements, courses, and attachments.
All cadavers were legally donated to the Catholic Kwandong University College of Medicine, and this study was conducted in accordance with the Declaration of Helsinki. No transplant donors were from a vulnerable population and all donors or their next of kin volunteered written informed consent. This study was approved by the Institutional Review Board of the Catholic Kwandong University (IRB No. CKU-21-01-0303).