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P53 luc plasmid

Manufactured by Agilent Technologies
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The P53-Luc plasmid is a laboratory tool used for the detection and measurement of p53 transcriptional activity. It contains a p53-responsive promoter that drives the expression of the luciferase reporter gene, allowing for the assessment of p53 function in cells.

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Lab products found in correlation

Prl tk renilla vector, by Promega (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Luciferase assay system, by Promega (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Close spelling variants of this product

P53-Luc Cis-reporter plasmid P53-Luc

2 protocols using p53 luc plasmid

1

Luciferase Assay for p53 Activity

Cited 1 time
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A549 cells were cotransfected with p53-Luc plasmid (Agilent Technologies, Santa Clara, CA, USA) and pRL-TK Renilla vector (Promega, Madison, WI, USA) using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). After 24 h, the transfected cells were treated with passive lysis buffer at room temperature for 10 min. Cell lysates were then transferred to a plate, and luciferase assay reagent and stop reagent were added in sequence. Next, luciferase activity was measured using a Luciferase Assay System (Promega, Madison, WI, USA) according to the manufacturer’s instructions, as previously described31 (link).
Jiang W., Jin G., Cai F., Chen X., Cao N., Zhang X., Liu J., Chen F., Wang F., Dong W., Zhuang H, & Hua Z.C. (2019). Extracellular signal-regulated kinase 5 increases radioresistance of lung cancer cells by enhancing the DNA damage response. Experimental & Molecular Medicine, 51(2), 1-20.
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2

Dual-Luciferase Assay for p53 Activity

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Cells were co-transfected with p53-Luc Plasmid (Agilent Technologies, Santa Clara, CA) and pRL-TK Renilla Vector (Promega, Madison, WI) using Lipofectamine 2000 (Life Technologies, Grand Island, NY). After 24 h, total cell extracts were assayed for luciferase activity using Dual-Luciferase® Reporter Assay System (Promega), and a multiwell plate luminometer (Turner Biosystems, Sunnyvale, CA) according to the manufacturer’s instruction.
Takahashi K., Yan I.K., Kogure T., Haga H, & Patel T. (2014). Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer. FEBS Open Bio, 4, 458-467.
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