Odyssey goat anti rabbit irdye 800 cw antibody

Western blot analysis was performed with 50 µg of protein extract. Antibodies were as follows: rabbit anti-iNOS polyclonal antibody (1:200, St. Louis, MO, USA), rabbit anti-NF-κB p65 polyclonal antibody (1:1,000), rabbit anti-ERK1/2 polyclonal antibody (1:1,000), and rabbit anti-phorspho-ERK1/2 polyclonal antibody (1:1,000) (Cell Signaling, Beverly, MA, USA); rabbit anti-β-tubulin and anti-GAPDH monoclonal antibodies (1:1,000, Abcam, UK). ODYSSEY goat anti-rabbit IRDye^® 800 CW antibody (1:10,000, LI-COR, NE, USA) was used as secondary antibody. The bands were displayed using ODYSSEY and quantified by Odyssey v3.0 software. β-tubulin or GAPDH was as reference.

Western blot analysis was performed with 50 μg of protein extract from cells or 100 μg from liver tissue, using monoclonal antibodies: citrullinated-histone H3 (1:1000, Abcam, ab5103); ERK1/2(1:1000, Cell Signaling, 4695S) and phosphor-ERK1/2 (1:1000, Cell Signaling, 4376S), p38 (1:1000, Cell Signaling, 9212S), and phosphor-p38 (1:1000, Cell Signaling, 9211S); caspase-3 (1:1000, Cell Signaling, 9662S) and cleaved caspase-3 (1:1000, Cell Signaling, 9661S); anti-β-tubulin (1:5000, Cell Signaling, 2146S) and anti-β-actin monoclonal antibodies (1:5000, Cell Signaling, 3700S). ODYSSEY goat anti-rabbit IRDye® 800 CW antibody and goat anti-mouse IRDye® 800 CW antibody (1:10,000, LI-COR) were used as secondary antibodies. The bands were displayed using ODYSSEY and quantified by Odyssey v3.0 software. β-Tubulin or β-actin was used as reference.