Catechol

Luria–Bertani (LB) broth, agar, catechol, veratryl alcohol, magnesium sulfate, zinc sulfate, copper sulfate, ammonium sulfate, silver chloride, mercuric chloride, calcium chloride, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, silver nitrate, sodium nitrate, potassium nitrate, ammonium nitrate and sodium thiosulfate, sulfanilamide, N-(1-naphthyl)ethylenediamine dihydrochloride (NEDD), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Diethyl aminoethyl (DEAE)-cellulose and Biogel P-150 were procured from Himedia (Mumbai, India). Coomassie brilliant blue R-250, hydrogen peroxide and n-propanol were obtained from Loba Chemie (Mumbai, India), SD Fine (Mumbai, India) and Qualigens (Mumbai, India), respectively. Ammonium sulphate, NADH, L-DOPA, guaiacol, hydroxyquinolone and tartaric acid were supplied by SRL Pvt Ltd (Mumbai, India).

Salicylic acid, hydrogen peroxide, catechol, guaiacol, polyvinylpyrrolidone, tris buffer, sodium phosphate, and di-sodium phosphate were purchased from Himedia, Bangalore, India. All chemicals used were of analytical grade (≥99% purity).

Guaiacol, 2, 2′-azino bis[3-ethylbenzthiazoline-6-sulfonate] (ABTS), syringaldazine (SGZ), sephadex G-150 and DEAE-cellulose were purchased from Sigma (USA). Other substrates viz. pyrogallol, α-naphthol, p-phenylenediamine (PPD), resorcinol, 2, 6-dimethoxyphenol (DMP), catechol, 3,4-dihydroxy-phenylalanine (L-DOPA) and tyrosine were purchased from Hi-media. Other chemicals used were of analytical grade.

The diazo dye Congo red (C.I. No. 22120, Direct red 28, MF: C₃₂H₂₂N₆Na₂O₆S₂ and FW: 696.66 g·mol⁻¹) which is the sodium salt of benzidinediazo-bis-1-naphthylamine-4-sulfonic acid, used in this study was supplied by Sigma-Aldrich (St. Louis, MO, USA). RTE was collected from Mahesh Textile Processors (Ichalkaranji, MS, India) in an airtight plastic can, passed through Whatman grade No. 1 filter paper to remove large suspended matter and stored in a refrigerator at 4 ± 1 °C until further use. Veratryl alcohol, catechol, methyl red, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nicotinamide adenine dinucleotide (NADH), dichlorophenol indophenol (DCIP), glucose, yeast extract and peptone were purchased from HiMedia Laboratories Pvt. Ltd. (Mumbai, MS, India). All of the chemicals used in this study were of analytical grade, unless otherwise mentioned. WB medium was prepared, the pH adjusted to 7.5 with with 0.1 N NaOH or 0.1 N HCL and used as growth medium for the biodegradation of dye and textile effluent [22 (link)].