Foxp3 transcription factor staining buffer set for nuclear proteins

Manufactured by Thermo Fisher Scientific

The Foxp3/Transcription Factor Staining Buffer Set is a laboratory reagent designed for the detection and analysis of nuclear proteins. The set includes buffers for fixation, permeabilization, and staining to facilitate the intracellular labeling of transcription factors and other nuclear proteins.

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Lab products found in correlation

Brefeldin a, by Thermo Fisher Scientific (2 mentions) Fixation and intracellular staining permeabilization wash buffers for cytoplasmic, by Sony (2 mentions) Daf fm staining, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Foxp3/Transcription Factor Staining Buffer Set (1238 citations) Foxp3 Staining Buffer Set (432 citations) Transcription Factor Staining Buffer Set (101 citations) Foxp3/Transcription Factor Staining Buffer (99 citations) Foxp3/transcription factor buffer set (37 citations) Foxp3/Transcription factor staining set (16 citations) Transcription factor staining buffer (16 citations) FoxP3 Transcription Staining Buffer Set (7 citations) Transcription factor buffer set (5 citations) FoxP3/transcription buffer set (4 citations)

2 protocols using foxp3 transcription factor staining buffer set for nuclear proteins

Multiparametric Flow Cytometry Staining

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Cells were stained with primary and secondary antibodies in pre-determined dilutions at 4 °C for 15 min (see Supplementary Table 3). Intracellular antigens were stained after cell surface antigens, using Sony’s Fixation and Intracellular Staining Permeabilization Wash Buffers for cytoplasmic, (Sony, 2704005, 2705010), and eBioscience’s Foxp3/Transcription Factor Staining Buffer Set for nuclear proteins (Thermo, 00-5523-00). Cytokines and secretory molecules were analyzed after Brefeldin A treatment (eBioscience, 00-4506-51); 5 µg/ml for 2 h at 37 °C before staining. Intracellular nitric oxide (NO) levels were determined using DAF-FM staining (Thermo Fisher, D23844), as recommended by the manufacturer.

Érsek B., Silló P., Cakir U., Molnár V., Bencsik A., Mayer B., Mezey E., Kárpáti S., Pós Z, & Németh K. (2020). Melanoma-associated fibroblasts impair CD8+ T cell function and modify expression of immune checkpoint regulators via increased arginase activity. Cellular and Molecular Life Sciences, 78(2), 661-673.

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Comprehensive Cell Immunophenotyping Protocol

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Cells were stained with primary and secondary antibodies in pre-determined dilutions at 4°C for 15 min (see Supplementary table 3). Intracellular antigens were stained after cell surface antigens, using Sony’s Fixation and Intracellular Staining Permeabilization Wash Buffers for cytoplasmic, (Sony, 2704005, 2705010), and eBioscience’s Foxp3 / Transcription Factor Staining Buffer Set for nuclear proteins (Thermo, 00–5523-00). Cytokines and secretory molecules were analyzed after Brefeldin A treatment (eBioscience, 00–4506-51); 5 μg/ml for 2 hours at 37°C before staining. Intracellular nitric oxide (NO) levels were determined using DAF-FM staining (Thermo Fisher, D23844), as recommended by the manufacturer.

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