Flow cytometry analysis with surface markers was conducted as described previously with some modifications (Su et al., 2018 (link)). Retinoic acid (R2625, Sigma-Aldrich) was utilized to induced myeloid differentiation in NB4 cells. Antibodies used included anti-mouse CD45.1 APC (17-0453-82, eBioscience), anti-mouse CD45.2-PE (12-0454-83, Thermo Fisher Scientific), anti-mouse CD117 (c-Kit) FITC (17-1171-82, eBioscience), anti-Human CD33 PE (12-0339-42, Thermo Fisher Scientific), anti-Human CD45 BV786 (563716, BD Horizon), PE anti-mouse/human CD11b antibody (12-0118-42, eBioscience), anti-Human CD15 APC (17-0158-42, eBioscience), anti-Human CD14 APC (17-0149-42, eBioscience), anti-Human LILRB4 PE (333008, BioLegend), anti-Human LILRB4 APC (17-5139-42, eBioscience), anti-human CD209 FITC (330103, BioLegend), anti-human CD86 PE (374205, BioLegend), anti-Mouse Lilrb4 Alexa Fluor 647 (144906, BioLegend), and anti-Human CD34 FITC (11-0349-42, eBioscience).
Anti human cd34 fitc
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-Human CD34 FITC is a fluorescent-labeled monoclonal antibody that binds to the CD34 antigen expressed on human hematopoietic progenitor cells. It is used for the identification and enumeration of CD34+ cells in flow cytometry applications.
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Lab products found in correlation
Anti mouse cd45.1 apc, by Thermo Fisher Scientific (1 mentions)
Anti human cd14 apc, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd117 c kit fitc, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd45.2 pe, by Thermo Fisher Scientific (1 mentions)
Pe anti human cd86, by BioLegend (1 mentions)
Retinoic acid, by Merck Group (1 mentions)
Fitc mouse anti human cd44, by BD (1 mentions)
Anti human cd31 fitc, by BD (1 mentions)
Calibur flow cytometer, by BD (1 mentions)
3 protocols using anti human cd34 fitc
1
Myeloid Differentiation Analysis via Flow Cytometry
2
Flow Cytometry Analysis of ADSC Purity
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The ADSCs from passage 3 were digested with 0.25% trypsin, and 1 × 106 cells were incubated with the following antibodies: Anti-Human CD34 FITC (1:1000; eBioscience), Anti-Human CD29 APC (1:1000; eBioscience), and FITC Mouse Anti-Human CD44 (1:1000; BD Pharmingen). Cell purity was determined by surface marker detection using flow cytometry24 (link).
3
Isolation and Characterization of Umbilical Cord-Derived MSCs
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Human umbilical cord‐derived MSC were isolated according to the previously described protocol. After receiving the appropriate written consent, MSC harvested from full‐term delivery UCs. The isolated MSCs were cultured with Mesenchymal Expansion Medium (R&D Systems Inc., Minneapolis, MN, USA) in a 5% CO2 incubator at 37°C. For flow cytometric analysis, the cells were incubated with the following antibodies: anti‐human CD105‐PE (eBioscience Inc., CA, USA), anti‐human CD34‐FITC (eBioscience Inc. CA, USA), anti‐human CD31‐FITC (BD Pharmingen Inc., San Diego, CA, USA). Then, the cells were washed with PBS and were analysed with a Calibur flow cytometer (BD Pharmingen Inc.).
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