Brown norway rats

Procedures concerning animals were performed in accordance with the EU Directive 2010/63/EU for animal experiments and with permission of the Dutch Central Authority for Scientific Procedures on Animals (CCD), permit number 1160020172924, approved 18, January, 2018. The animals were maintained on a 12 h day–night cycle and were supplied ad libitum with food and water. Brown Norway rats from Janvier Labs with a spontaneous mutation in the Crb1 gene were used in this study [10 (link)]; the Crb1 mutant rat breeding was set up within the LUMC animal facility. Age-matched control Brown Norway rats lacking the mutation in the Crb1 gene from Charles River Laboratories were used as controls. Animals were killed by carbon dioxide inhalation.

Fifty six 10 week-old male Brown Norway rats weighting 250–300 g (Janvier, Le Genest-St-Isle, France) were sensitized by subcutaneous administration of chicken egg albumin as previously described [15 (link),16 (link)]. Animals were randomized between control (CON, n = 28) and shocked (AS, n = 28) groups. Hemodynamics were measured in all animals (n = 56).
Twenty eight animals were used to study mitochondrial respiration and ROS production; 14 animals were used to study superoxide dismutase (SOD) activity and reactive nitrogen species (RNS) production; and the 14 remaining rats were used to study oxidative damage, in the CON group (n = 7) and in the AS group (n = 7) for each experimentation.
AS was induced by an intravenous injection of 1 mg ovalbumin diluted in 1 mL of 0.9% saline solution (T0). In the control group, 1 mL of normal saline solution was injected (T0).
All animal procedures and care were performed in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC). The study protocol was approved by the Ethical Committee for Animal Experiments of Strasbourg University, France (N° #16987-2018100500047648).