Anti cd40 antibody clone fgk4.5

Spleen cells were isolated from BC10.3 mice or 6C2.16 × BALB/c F1 hybrids, and 2 × 10⁷ spleen cells per mouse were adoptively transferred into MHC class I–matched HBV-Tg or non-Tg recipients. In selected experiments, CD8⁺ cells were purified from the spleen cells of BC10.3 mice by negative selection using MACS (Miltenyi Biotec) according to the manufacturer’s instructions, and 3 × 10⁶ CD8⁺ T cells were adoptively transferred into each recipient. Effector HBV-specific CD8⁺ T cells were generated by adoptively transferring COR93-specific naive CD8⁺ T cells into B6 mice that were then immunized with COR93 peptide (purchased from Scrum) and a monoclonal anti-CD40 antibody (clone FGK4.5; catalog BE0016-2, Bio X Cell).