Anti cd11b alexa fluor700 clone m1 70

Manufactured by BioLegend

Anti-CD11b Alexa Fluor700 (clone M1/70) is a fluorescently-labeled monoclonal antibody that binds to the CD11b cell surface antigen. CD11b is a component of the Mac-1 integrin and is expressed on the surface of myeloid cells, including monocytes, macrophages, and granulocytes.

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Lab products found in correlation

Facsaria 3, by BD (1 mentions) 5 bromo 2 deoxyuridine brdu, by Thermo Fisher Scientific (1 mentions) Efluor450 cocktail, by Thermo Fisher Scientific (1 mentions) Brdu staining kit, by Thermo Fisher Scientific (1 mentions) Diff quik, by Siemens (1 mentions)

Spelling variants of this product

CD11b (457 citations) Anti-CD11b (179 citations) CD11b (M1/70, (86 citations) CD11b (clone M1/70, (72 citations) M1/70 (46 citations) Anti-CD11b (clone M1/70, (45 citations) Anti-CD11b (M1/70, (42 citations) Clone M1/70 (26 citations)

2 protocols using anti cd11b alexa fluor700 clone m1 70

Tumor-associated Macrophage Isolation

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Single-cell suspensions obtained from tumors were stained with DAPI, anti-CD45 APC (clone I3/2.3, Biolegend #147708) and anti-CD11b Alexa Fluor700 (clone M1/70, Biolegend #101222) and subjected to FACS using a FACS Aria III (BD). Live cells were identified as DAPI negative and TAMs as CD45⁺ CD11b⁺F4/80⁺ populations.

Kim S.H., Saeidi S., Zhong X., Gwak S.Y., Muna I.A., Park S.A., Kim S.H., Na H.K., Joe Y., Chung H.T., Kim K.E., Han W, & Surh Y.J. (2020). Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages. Neoplasia (New York, N.Y.), 22(11), 606-616.

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Bone Marrow Cell Characterization

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BM cells from femur and tibia were collected, enumerated, and differential counts were performed on Diff-Quik^™ (Siemens)-stained slides (26 (link), 58 (link)). For FACS analyses (29 (link)), antibodies were: anti-CD11b (AlexaFluor700, clone M1/70, BioLegend); anti-Ly-6G/6C (APC-Cy7, clone RB6-8C5, Pharmingen); mouse hematopoietic lineage marker mix (eFluor 450 cocktail, eBioscience), anti-ckit (APC, clone 2B8, eBioscience); and anti-Sca-1 (PE, clone D7, eBioscience). Caspase-3 activity was analyzed using CaspGLOW^™ Red Active Caspase staining reagents (Biovison) in combination with cell surface marker analysis (29 (link)). Data were acquired using an LSR FACS (Becton Dickinson) and analyzed using FlowJo Software. For analysis of proliferative responses, mice received 100 mg/kg body weight of 5-bromo-2′deoxyuridine (BrdU, Fisher Scientific) via intra-peritoneal injection two days before harvest (17 (link)) and cells were analyzed using a BrdU-Staining kit (eBioscience).

Prigge J.R., Hoyt T.R., Dobrinen E., Capecchi M.R., Schmidt E.E, & Meissner N. (2015). Type-I-IFNs act upon hematopoietic progenitors to protect and maintain hematopoiesis during Pneumocystis lung infection in mice. Journal of immunology (Baltimore, Md. : 1950), 195(11), 5347-5357.

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