For the microbiological analysis, samples of 5 g of disinfected larvae were collected and diluted tenfold in physiological peptone solution (PPS, 0.85% NaCl, 0.01% peptone) before pulverizing the larvae in the solution by using an ethanol sterilized home type mixer (Bosch CNHR 25). Similarly, 5 g of substrate was sampled from each replicate and diluted 1:10 in PPS. Next, both larval and substrate samples were homogenized using a stomacher (BagMixer 400CC, Interscience, France) for 1 min. For each sample, the total aerobic viable count and the specific Salmonella count were determined. All plate counts were performed according to the ISO standards for microbial analyses of food and feed as compiled by Dijk et al. (2015) . For the total aerobic viable count, serial dilutions were made in PPS, plated on Plate Count Agar (PCA, Biokar Diagnostics, Beauvais, France), and incubated at 30 °C for 72 h. Salmonella was counted by plating the diluted samples on a chromogenic RAPID′ Salmonella agar (BioRad Laboratories, Belgium) and incubating the plates at 37 °C for 24 h. The cell density of all inocula was also verified by plating a serial dilution on both the RAPID’ Salmonella agar and PCA.
Bagmixer 400cc
Manufactured by Interscience
Sourced in France
The BagMixer 400CC is a laboratory homogenizer designed for the efficient mixing and blending of samples. It features a compact and robust construction, with a capacity of up to 400 mL. The BagMixer 400CC utilizes a controlled, high-speed mixing action to ensure thorough and consistent sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
Half fraser broth, by Merck Group (2 mentions)
Fraser broth, by Merck Group (2 mentions)
Cnhr 25, by Bosch (1 mentions)
Rapid salmonella agar, by Bio-Rad (1 mentions)
Plate count agar pca, by Solabia (1 mentions)
Peptone, by BD (1 mentions)
Genbox anaer, by bioMérieux (1 mentions)
Maldi tof mass spectrometer, by Bruker (1 mentions)
Mp220, by Mettler Toledo (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
11 protocols using bagmixer 400cc
1
Microbiological Analysis of Disinfected Larvae
2
Quantification of Clostridium perfringens in Chicken Cecal Contents
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On days 4, 5 and 6 after Eimeria challenge, 11 chickens per treatment group (1 chicken from each replicate pen) were randomly selected and humanely euthanized by cranial stunning immediately followed by cervical dislocation before necropsy. Samples of cecal contents were collected in sterile stomacher bags and directly subjected to cultivation in order to quantify CP. In brief, the samples were diluted 1:100 in peptone saline water (0.1% peptone, Difco Laboratories Inc., Detroit, US and 0.85% NaCl) and homogenized for 30 s in a stomacher (Bagmixer 400 CC, Interscience, Saint Nom, France). Serial dilutions were made with non-buffered peptone water until a dilution of 10–6 was reached. Aliquots of 100 µL from the dilutions 10–2, 10–4 and 10–6 were plated onto sheep blood agar plates (Oxoid Blood Agar Base No.2 and 5% sheep blood, manufactured by the Norwegian Veterinary Institute, Oslo, Norway). The plates were incubated anaerobically at 37 °C for 24 h (Genbox anaer, Biomérieux, Marcy-l’Étoile, France). Single colonies with double hemolysis were counted, and colony-forming units per gram (cfu/g) cecal contents were calculated based on the given dilution. Typical colonies were selected for pure cultivation and later confirmed as CP by a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer (Bruker Daltonics, Bruker Corp., Billerica, MA, USA).
3
Microbial Analysis of Stored Bamboo Sprouts
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The microbial growth in stored sprouts was analyzed following the method of Li et al. [25 (link)], with slight modifications. Sampling sprouts were performed under aseptic conditions. Bamboo sprouts (10 g) and sterile Ringer’s solution (90 mL) were homogenized in a stomacher bag filter and homogenized with a Bag Mixer 400CC (Interscience, Puycapel, France). An aliquot of each dilution was plated in triplicate on various selective agars. The total bacterial count (TBC) was determined in Plant Count Agar, Oxoid, and incubated at 25 ± 2 °C for 48 h. The analysis of yeast and molds required DRBC (Dichloran Rose Bengal Chloramphenicol) agar base plates and an incubation time of 48 h at 28 ± 2 °C. Microbiological data (TBC) and total yeast and mold count (TYMC) were expressed as the logarithms of colony-forming units per gram (log CFU/g). Each test was performed in triplicate.
4
Mackerel Total Viable Count Protocol
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TVC was measured using the dry film method [27 ]. Ten grams of mackerel were collected, put in a stomacher bag, mixed with sterile peptone saline buffer (Difco, Sparks, MD, USA), and homogenized for 1 min using a stomacher (BagMixer® 400 CC, Interscience, Saint-Nom-la-Bretèche, France). After appropriately diluting, 1 mL of each dilution solution was inoculated on three sheets of dry film medium, and the number of red colonies was measured after 48 h 35 °C in an incubator. TVC was calculated.
5
Microbial Enumeration of Sausage Samples
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Homogenized sausage sample (10 g) and double-distilled (dd) water (90 mL) were
mixed with a stomacher (BagMixer® 400 CC®,
Interscience, Saint-Nom-la-Bretèche, France) for microbial counts. Total
plate count (TPC) and violet red bile (VRB) agar plates were used for measuring
numbers of total bacteria and Enterobacteriaceae, respectively. After spreading
samples onto agar plates in petri dishes, plates were incubated at 37°C
in an incubator for 48 h.
mixed with a stomacher (BagMixer® 400 CC®,
Interscience, Saint-Nom-la-Bretèche, France) for microbial counts. Total
plate count (TPC) and violet red bile (VRB) agar plates were used for measuring
numbers of total bacteria and Enterobacteriaceae, respectively. After spreading
samples onto agar plates in petri dishes, plates were incubated at 37°C
in an incubator for 48 h.
6
Quality Assessment of Shrimp Samples
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The TVB‐N content was determined according to Chen, Jiao, Yu, et al. (2022 ). The results were expressed in mg N/100 g of sample. Based on the Chinese National Standard (GB2733‐2015), the limit of TVB‐N values was set as 30 mg N/100 g for shrimp.
A sample of 1 g was homogenized with 9 ml of water distilled CO2 free at 12,000 rpm for 30 s. The pH value was measured using a pH meter (MP220, Mettler Toledo) at 20°C ± 2°C.
The TBARS of shrimps was performed as described by (John et al., 2005 (link)). The results were expressed as mg MDA/kg of sample.
The total aerobic viable count of shrimps was determined. Samples (10 g) were mashed and transferred to a sterile bag (Bkmam®), and then diluted 10 times sterile water and homogenized (BagMixer 400CC, Inter Science) for 2 min. Appropriate serial dilutions of homogenates (0.1 ml each) were spread on sterile agar plates. After pouring the media, the plates were cultured for 72 h at 30°C and the bacterial counts were enumerated.
A sample of 1 g was homogenized with 9 ml of water distilled CO2 free at 12,000 rpm for 30 s. The pH value was measured using a pH meter (MP220, Mettler Toledo) at 20°C ± 2°C.
The TBARS of shrimps was performed as described by (John et al., 2005 (link)). The results were expressed as mg MDA/kg of sample.
The total aerobic viable count of shrimps was determined. Samples (10 g) were mashed and transferred to a sterile bag (Bkmam®), and then diluted 10 times sterile water and homogenized (BagMixer 400CC, Inter Science) for 2 min. Appropriate serial dilutions of homogenates (0.1 ml each) were spread on sterile agar plates. After pouring the media, the plates were cultured for 72 h at 30°C and the bacterial counts were enumerated.
7
Homogenization and Centrifugation of Food Samples
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In total, 10 g of each sample were homogenized 120 s with 90 ml sterile 1 × phosphate buffered saline (PBS, Gibco, Bleiswijk, The Netherlands) in sterile Stomacher®400 classic strainer bags (Seward Ltd, Worthing, United Kingdom) using a BagMixer®400 CC (Interscience, Puycapel, France). To remove coarse food particles, the homogenates were centrifuged at 300 × rcf (relative centrifugal force) for 2 min at room temperature (RT) using an Eppendorf Centrifuge 5810R and an A-4-62 rotor (Eppendorf Corporate, Hamburg, Germany). The remaining supernatants were transferred to new tubes and centrifuged at 3000 × rcf (30 min at RT). The obtained cell pellets were diluted 1:10 (v/v) with sterile 1 × PBS and were used freshly for bacterial and fungal isolation or were frozen at −20 °C for later cultivation approaches and at −80 °C for DNA extraction.
8
pH and Moisture Content Determination
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The pH measurement was carried out in accordance with the previous report (Ruiz-Capillas et al., 2012 (link)) with some modifications. Samples (5 g each) were dissected and homogenized in 90 mL of 0.85% saline using a BagMixer (400CC; Interscience, Saint-Nom la Bretèche, France) for 90 s. The pH of the filtrate was measured using a pH meter (Testo 206-PH1; Testo AG, Lenzkirch, Germany). The water content was estimated in accordance with previous analytical methods (Adam et al., 2009 ) with minor modifications, and samples (20 g each) were placed in a dry evaporation pan and dried to a constant weight at 106°C for about 5 h. The water content was calculated based on the difference between the weights of the initial sample and dried sample.
9
Microbial Enumeration in Food Samples
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Total mesophilic aerobic bacteria count, yeast and mold count analyses were done according to methods (990:12; 997:12) in Anonymous (2012a ,b ). In both analyses, 10 g of each thawed sample was taken into sterile bags and homogenized in a stomacher (BagMixer 400 CC, Interscience, France) by adding 90 ml of 0.1% peptone water. Suitable 3M Petrifilm medium plates (3M Cooperation, USA) were used for growth medium.
10
Isolation of Listeria monocytogenes from Food
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Each fresh sample was weighted in 25 g and the isolation of L. monocytogenes was conducted according to the classic culture method. This method includes two steps of pre-enrichment and selective enrichment in Half Fraser and Fraser Broth, respectively. After 225 ml of Half Fraser Broth (Merck 1.10398.0500) were added to samples (25 g), samples were homogenized in a stomacher (Interscience BagMixer 400 cc, France) for 2 to 3 min. The obtained homogenate was incubated for 24 h at 30°C for pre-enrichment. Following the pre-enrichment, 0.1 ml were taken from the culture and transferred into tubes containing 10 ml of Fraser Broth (Merck 1.10398.0500), then they were incubated for 24 h at 37°C. Inoculation into PALCAM Agar (Polymyxin Acriflavine Lithium Chloride Ceftazidime Aesculin Mannitol Agar, Oxoid CM0877-SR0150E) was conducted and agar plates were left for incubation at 37°C for 48 h. At the end of the incubation period, 1-5 Listeria suspected colonies (black-centered, gray green in color with a black halo) in PALCAM Agar were selected and were transferred into TSA-YE (Tryptone Soy Agar with Yeast Extract, Oxoid CM0131-YE). TSA-YE grown colonies were transferred to TSB (Tryptone Soy Broth, Oxoid CM0129) containing 20% glycerin and were stored in -20°C for advanced analyses (Jeyaletchumi et al., 2010) .
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