In Situ Hybridization (ISH) was conducted using an In situ hybridization kit (Boster, China). Tissue sections were processed to de-paraffinization, dehydration and digested with pepsin. Subsequently, sections were incubated with FAM225A-specific probe at 50°C overnight. Sections were further incubated with biotinylated anti-Digoxin (Boster, China), stained with biotinylated peroxidase, and visualized with 3,3'-diaminobenzidine substrate.
Biotinylated anti digoxin
Manufactured by Boster Bio
Sourced in China
The Biotinylated anti-Digoxin is a laboratory reagent used for the detection and quantification of the cardiac glycoside Digoxin in biological samples. It functions as a specific and sensitive binding agent for Digoxin, enabling its identification and measurement through various analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biotinylated anti digoxin
1
In Situ Hybridization for FAM225A Detection
2
In Situ Hybridization of circ_0016070
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In situ hybridization staining was performed using an in situ hybridization kit (Boster, China). PH tissue sections were deparaffinized, dehydrated, and pepsin‐digested. Samples were incubated with circ_0016070‐specific probe overnight at 50 °C and further incubated with biotinylated antidigoxin (Boster, China), stained with biotinylated peroxidase, and developed with 3,3′‐diaminobenzidine substrate. Then, the sections were observed by using a light microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!