The expression of glucagon-like peptide-1 (GLP-1) in the terminal ileum of rats was analyzed through an immunochemistry assay. Briefly, the tissues of the terminal ileum were first incubated with 4% tissue fixative, xylene, and gradient alcohol. Then, the tissues were embedded into the paraffin and cut into 4 μm tissue slices, followed by dewaxing and incubation with antigen retrieval buffer (C1032, Solarbio) and goat serum (16210064, Thermo, Waltham, Massachusetts, USA). Subsequently, the tissue slices were cultivated with anti-GLP-1 antibody (ab111125, Abcam, Cambridge, UK) at 4 °C overnight and further incubated with rabbit IgG (ab172730, Abcam) at room temperature for 1 h. Next, the tissue slices were stained with DBA buffer (SFQ004, 4 A Biotech, Beijing, China). After that, the tissue slices were further colored with hematoxylin and incubated with gradient alcohol, xylene, and neutral gum. Finally, the GLP-1 positive cells in the tissue slices were observed using the THUNDER tissue microscope with magnification × 100.
Sfq004
Manufactured by 4A Biotech
Sourced in China
The SFQ004 is a laboratory equipment designed for scientific research and analysis. It is a compact and versatile instrument that can perform a variety of tasks essential for various scientific disciplines. The core function of the SFQ004 is to facilitate efficient and accurate data collection and processing in controlled laboratory environments.
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Lab products found in correlation
3 protocols using sfq004
1
Immunochemical Analysis of Ileal GLP-1 Expression
2
Immunohistochemical Analysis of GNPNAT1 Expression in Murine Tumors
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The expression of GNPNAT1 in mice tumor tissues was analyzed through immunochemistry assay. Briefly, the collected tumor tissue was fixed with 4% tissue fixative (P1110, Solarbio), dehydrated with gradient alcohol, and then transparently treated with xylene (50,009, Meryer). Subsequently, the tissue was embedded into paraffin (M27079, Meryer) and sectioned into 4 μm slices. After being dewaxed, the tissue slices were added with antigen retrieval buffer (C1032, Solarbio) and goat serum (16,210,064, Thermo Fisher Scientific). Subsequently, the tissue slices were incubated with anti‐GNPNAT1 antibody (ab234981, Abcam) at 4°C overnight, followed by further incubation with goat anti‐rabbit IgG (ab205718, Abcam) for 1 h. Then, the tissue slices were colored by DBA buffer (SFQ004, 4A Biotech, Beijing, China) and haematoxylin (H8070, Solarbio) and further treated with gradient alcohol, xylene, and neutral gum (G8590, Solarbio). Lastly, the GNPNAT1‐positive cells in the tissue slices were observed using a THUNDER tissue microscope at a magnification of 100 × .
3
Immunohistochemical Analysis of PD-L1 Expression
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The tumor tissues of mice were fixed with 4% paraformaldehyde and made transparent by rxylene (ALFL13317, OKA). Next, the tissues were embedded into paraffin (A56132, OKA), sectioned into 4 μm, and dewaxed. After soaking in antigen repair buffer (R20902, OKA) for 8 min, the tissues were further incubated with the antibody of PD-L1 (13684, CST) overnight at 4°C, soaked in goat-anti-rabbit antibody (ab205718, Abcam) for 1 h, and further dyed with DBA (SFQ004, 4A Biotech, Beijing, China). After washing the tissues under tap water, the tissues were then dyed with hematoxylin (D10519, OKA) for 4 min and further made transparent by xylene. Finally, the expression image of PD-L1 in the tumor tissue was observed under a DMLA full automatic microscope (Leica, Solms, Germany).
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