Itgav hs00233808 m1

Manufactured by Thermo Fisher Scientific

Sourced in Germany

ITGAV Hs00233808_m1 is a TaqMan Gene Expression Assay designed to detect and quantify the expression of the integrin subunit alpha V (ITGAV) gene. This assay provides a tool for researchers to investigate the expression levels of the ITGAV gene, which is involved in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Itgav (2 citations)

2 protocols using itgav hs00233808 m1

Quantitative Real-Time PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted 72 h after transfection using the innuPREP RNA Mini Kit (Analytik Jena AG, Jena Germany) according to the manufacturer’s instructions. In total, 1 µg of total RNA was reverse-transcribed using the High-Capacity RNA-to-cDNA kit and microRNA was reverse-transcribed into cDNA using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Darmstadt, Germany). RNU6B was used as the reference value. qPCR was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems, Darmstadt, Germany) with 100 ng cDNA per 20 µL. The 18S or RNU6B was used as the reference housekeeping gene. The primers were purchased from Applied Biosystems (Darmstadt, Germany) (ITGAV Hs00233808_m1, hsa-mir-142-3p TM 000464, CFL2 Hs01071313_g1, RAC1 Hs01902432_s1, ROCK2 Hs00153074_m1, WASL Hs00187614_m1, RNU6B TM 001093, and 18S Hs99999901_s1). The data were analyzed using the delta-delta Ct method [30 (link)].

Börschel C.S., Stejskalova A., Schäfer S.D., Kiesel L, & Götte M. (2020). miR-142-3p Reduces the Size, Migration, and Contractility of Endometrial and Endometriotic Stromal Cells by Targeting Integrin- and Rho GTPase-Related Pathways That Regulate Cytoskeletal Function. Biomedicines, 8(8), 291.

+ Open protocol

+ Expand

RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated using Trizol reagent (Invitrogen), following manufacturer’s instructions. Briefly, the cells were lysed with Trizol, followed by phenol-chloroform phase separation. RNA was precipitated with isopropanol, washed with 75% ethanol and dried RNA pellet was resuspended in DEPC water. RNA from mouse tissue was isolated using RNeasy mini kit (Invitrogen), according to the instruction manual. cDNA was synthesized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Gene expression levels were assayed by RT-qPCR using 7900 HT FAST Real-Time PCR system, TaqMan Fast Universal Master Mix and TaqMan probes for specific genes (Applied Biosystems): ITGAV Hs00233808_m1, ITGB3 Hs00173978_m1, MYC Hs00153408_m1, SERPINE (PAI-1) Hs01126607_g1, GAPDH Hs99999905_m1. All assays were performed in triplicate and analysis was performed using RQ Manager software (Applied Biosystems) and the 2^−ΔΔCt method to obtain relative quantitation (RQ) values, with GAPDH used as endogenous control.

Cichon M.A., Moruzzi M.E., Shqau T.A., Miller E., Mehner C., Ethier S.P., Copland J.A., Radisky E.S, & Radisky D.C. (2016). MYC is a crucial mediator of TGFβ-induced invasion in basal breast cancer. Cancer research, 76(12), 3520-3530.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!