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Irtracer 100 ftir

Manufactured by Shimadzu
Sourced in Japan

The IRTracer-100 FTIR is a Fourier Transform Infrared Spectrometer manufactured by Shimadzu. It is designed to analyze the infrared absorption spectra of various samples. The core function of the IRTracer-100 FTIR is to measure the infrared radiation absorption and transmission characteristics of materials.

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9 protocols using irtracer 100 ftir

1

FTIR Spectroscopy of Dried Samples

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FTIR analysis was performed with an FTIR IRTracer-100 (SHIMADZU Corporation, Kyoto, Japan) from 400 to 4000 cm−1 with 32 scans and 4 cm−1 resolution. A dried sample (0.4 mg) was ground with KBr (40 mg) in an agate mortar, sheeted to one slice and scanned with a blank KBr background.
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2

FTIR Spectroscopy Analysis of Hydrogel

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Fourier Transform Infrared Spectroscopy (FTIR) is an analytical technique used to identify organic, polymeric, and in limited cases, in inorganic materials. FTIR test observes the functional groups of compounds, and in this study using the FTIR IRTracer-100 Shimadzu. First, hydrogel sample based on hyaluronic acid-chitosan to be mixed with KBr powder, put on a platinum pan and will be penetrated by infrared rays. The x-axis is referred to wave number of compound, and y axis is referred to the percentage transmittance. The functional group graph analysis is performed by correlate transmittance band at infrared spectrum and handling with the compound spectrum comparison [11 (link)].
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3

Analytical Characterization of Organic Compounds

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All solvents and reagents were obtained from Sigma-Aldrich (Castle Hill, Sydney, Australia) and used without any further purification or treatment. The THF for the fluorescence measurements specifically contained no fluorescent stabilizers. Thin layer chromatography was performed on silica gel plates from Grace Davison Discovery Sciences (US). Column chromatography was performed on a Grace Reveleris X2.
1 H and 13 C NMR spectra were obtained on a Varian 400MR at 400 MHz and 100 MHz, respectively.
Coupling constants (J) are in Hertz (Hz), and chemical shifts (δ) are expressed in parts per million (ppm) and reported relative to residual solvent peaks. Melting points were recorded on a Stuart Scientific SMP 10.
Infrared spectra were obtained on Shimadzu FTIR IR Tracer-100 and Shimadzu FTIR-8400S spectrophotometers.
Excitation and emission spectra were obtained on a Shimadzu RF-5301PC spectrophotometer. Microwave reactions were performed on CEM Discover. Low resolution mass spectra were obtained on Thermo Scientific TSQ Quantum Access Max LCMS/MS & TLX1 Turboflow Chromatography System in positive ion mode. High resolution mass spectra were generated on Bruker 7T Fourier Transform Ion Cyclotron
Resonance Mass Spectrometer (FTICR) in positive ion mode.
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4

FTIR Spectroscopy Protocol for Material Analysis

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FTIR analyses were carried out by using IRTracer-100
FTIR (Shimadzu, Japan), and 32 scans were captured for each spectrum
in a scanning array of 4000–400 cm–1 wave
numbers with resolution of 4 cm–1.28 (link)
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5

FTIR Analysis of Cabbage Leaf Composition

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For each experiment, relevant plant material was taken from the inner and outer leaves of cabbage and encoded with the letters “i” and “o”, respectively. Plant material was dried in an oven at 55 °C for 2 days and was then crushed and sifted through a 0.5 mm sieve. The fraction < 0.5 mm was further used for analyses. Fourier-transform infrared (FTIR) spectra were recorded with the attenuated total reflectance (ATR) technique on an IRTracer-100 FTIR (Shimadzu, Kyoto, Japan) in wavenumbers ranging from 4000 to 400 cm−1 by the accumulation of 45 spectra at a resolution of 4 cm−1. Commercial MCC, pectin from citrus peels, and lignin alkali were used as FTIR standard macromolecules. The exported files were processed and graphically overlaid using OriginPro software version 9 from OriginLab Corporation (Northampton, MA, USA).
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6

UATR-FTIR Spectroscopic Analysis of Solid Samples

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UATR-FTIR spectroscopic analysis was carried out using a Shimadzu IRTracer-100 FT-IR spectrometer with ATR device. The data were collected directly from solid samples in the spectral range 4000–400 cm−1. Spectra were built up after a number of 16 co-added acquisitions, with a spectral resolution of 2 cm−1.
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7

FTIR Analysis of Electrospun Fibers

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The electrospun fiber samples were then tested for functional groups using the Shimadzu IRTracer-100 FTIR at a wavenumber of 400–4000 cm−1. The FTIR test results show the relationship between % transmission and wavenumber spectrum (cm−1). The absorbance band formed in the sample's infrared spectrum was compared to standard data and the spectrum of the comparison compound was used for functional group analysis.
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8

Fourier Transform Infrared Analysis of Bone

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Fourier Transform Infrared Spectroscopy (FTIR) provides molecular-level insights that enable the analysis of functional groups, bonding types, and molecular conformations [25 (link)] of both native bone and tDBM. Infrared spectroscopy analysis was performed using an IRTracer-100 FTIR (Shimadzu, Kyoto, Japan) to investigate the chemical composition of the samples. The lyophilized samples were positioned onto the spectrum plate and scanned over a wavelength range spanning from 400 to 4000 cm−1. The absorbance of infrared wavelengths was automatically quantified by the software and displayed as a percentage in the Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectrum. The background was subtracted from the obtained spectra, and different peaks corresponding to the type I collagen and hydroxyapatite markers were identified.
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9

FTIR Analysis of PPP Reaction Product

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FTIR spectroscopy was used to investigate the chemical bonds present in the product of the reaction. Samples of PPP were analyzed using an ATR accessory sampling technique on a Shimadzu IRTracer-100 FTIR with a total of 20 scans, resolution of 4 cm−1, and wavenumber range of 4000-500 cm−1.
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