The antibodies α-Cpx (Troy Littleton, MIT, Cambridge, MA; Huntwork and Littleton, 2007 (link)) and α-phospho-Mad (PS1; Peter ten Dijke, Leiden University Medical Center, Leiden, Netherlands; Dan Vasiliauskas, Susan Morton, Tom Jessell, and Ed Laufer, Columbia University Medical Center, New York, NY; Persson et al., 1998 (link)) have been described previously. α-Rab11 antibodies were obtained from BD Biosciences, San Jose, CA (clone 47; Khodosh et al., 2006 (link)), α-TDP-43 antibodies (10782-2-AP) from Proteintech, Rosemont, IL, and α-tubulin antibodies (clone B-5-1-2) from Sigma-Aldrich, St. Louis, MO. Rhodamine Red-X– and Alexa 647-conjugated α–horseradish peroxidase (HRP) antibodies were obtained from Jackson ImmunoResearch, West Grove, PA. α-Dlg (4F3), α-Futsch (22c10), α-Wit (23C7), α-actin (JLA20), α-eve (2B8), and α-synapsin (3C11) antibodies were obtained from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA). Secondary antibodies for imaging were conjugated to Dylight 488 or Rhodamine Red-X (Jackson ImmunoResearch). Immunoblots were imaged using infrared dye–conjugated secondary antibodies (Rockland Immunochemicals, Pottstown, PA) on a LI-COR Odyssey device.
α tubulin antibodies clone b 5 1 2
Manufactured by Merck Group
α-tubulin antibodies (clone B-5-1-2) are monoclonal antibodies that recognize the α-tubulin protein. α-tubulin is a component of the microtubule cytoskeleton in eukaryotic cells. These antibodies can be used for the detection and localization of α-tubulin in various applications such as immunohistochemistry, immunocytochemistry, and Western blotting.
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2 protocols using α tubulin antibodies clone b 5 1 2
1
Comprehensive Antibody Database for Neuroscience Research
2
Western Blot Analysis of Drosophila Dap160
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Drosophila heads (10 pooled heads/genotype) were homogenized in 100 µl 2x Laemmli buffer. 10 ul of extract per lane was fractionated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with α-Dap160 (Roos and Kelly, 1998 (link)) and α-tubulin antibodies (clone B-5-1-2; Sigma), and infrared-conjugated secondary antibodies (Rockland, Inc). Blots were analyzed on a Biorad Chemidoc system.
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Drosophila heads (10 pooled heads/genotype) were homogenized in 100 µl 2x Laemmli buffer. 10 ul of extract per lane was fractionated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with α-Dap160 (Roos and Kelly, 1998 (link)) and α-tubulin antibodies (clone B-5-1-2; Sigma), and infrared-conjugated secondary antibodies (Rockland, Inc). Blots were analyzed on a Biorad Chemidoc system.
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