Non-fasting blood samples were collected using venipuncture techniques between 9 and 10 am. The separation of plasma and peripheral blood mononuclear cells (PBMCs) was performed within 24 h of blood sample collection using PBMC isolation tubes (STEMCELL Technologies, Vancouver, BC, Canada). PBMCs were stored in liquid nitrogen for further applications. Plasma was stored at −80 °C prior to assays.
Pbmc isolation tubes
Manufactured by STEMCELL
Sourced in Canada
PBMC isolation tubes are a specialized laboratory product designed to facilitate the isolation of peripheral blood mononuclear cells (PBMCs) from whole blood samples. These tubes contain a density gradient medium that allows for the separation of PBMCs from other blood components during centrifugation.
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Lab products found in correlation
2 protocols using pbmc isolation tubes
1
Blood Sample Processing for PBMC Isolation
2
Plasma and PBMC Isolation
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Blood was processed within 24 h of collection to separate plasma and PBMCs using PBMC isolation tubes (STEMCELL Technologies, Vancouver, BC, Canada). Plasma samples were kept at – 80 °C, and PBMCs at − 150 °C, until ready to assay.
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