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Socs1 overexpressing lentiviral plasmid

Manufactured by Hanbio Biotechnology

The SOCS1-overexpressing lentiviral plasmid is a recombinant DNA vector designed to deliver and express the Suppressor of Cytokine Signaling 1 (SOCS1) gene. SOCS1 is a key regulator of cytokine signaling pathways. The plasmid can be used to study the biological functions of SOCS1 in various cell lines and research applications.

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2 protocols using socs1 overexpressing lentiviral plasmid

1

siRNA Transfection and miRNA Regulation

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Stable transfection small interfering RNAs (siRNAs) were obtained from RiboBio (Guangzhou, China) and transfected into cells using Lipofectamine iMax (Invitrogen, AL, USA) following the manufacturer's instructions. The human lentivirus miR-331-3p sponge and the SOCS1-overexpressing lentiviral plasmid were purchased from HanBio. miRNA mimics and inhibitors were purchased from GenePharma (Shanghai, China). Transfection efficiency was verified using qRT-PCR.
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2

Lentiviral-mediated siRNA and miRNA Manipulation

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Stable transfection small interfering RNAs (siRNAs) were obtained from RiboBio (Guangzhou, China) and transfected into cells using Lipofectamine iMax (Invitrogen AL USA) following the manufacturer's instructions. The human lentivirus miR-331-3p sponge and the SOCS1-overexpressing lentiviral plasmid were purchased from HanBio. miRNA mimics and inhibitors were purchased from GenePharma (Shanghai, China). Transfection e ciency was veri ed using qRT-PCR.
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