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Anti cd3 mab 145.2c11

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The Anti-CD3 mAb (145.2C11) is a monoclonal antibody that binds to the CD3 complex on the surface of T cells. It is a commonly used tool in flow cytometry and cell biology research.

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Lab products found in correlation

Soluble anti cd28 37.51, by Cytek Biosciences (1 mentions) Mouse cd4 cd25 regulatory t cell isolation kit, by Miltenyi Biotec (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Interleukin 2 (il 2), by Immunotools (1 mentions)

Close spelling variants of this product

Anti-CD3 (145-2C11, CD3 (145–2C11, Anti-CD3

2 protocols using anti cd3 mab 145.2c11

1

Regulatory T Cell Suppression Assay

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CD4+CD25− (Tconv) and CD4+CD25+ (Treg) T cells were magnetically separated from LN specimens by autoMACS Pro Separator using the mouse CD4+CD25+ Regulatory T Cell Isolation Kit (Milteny Biotec). Upon CFSE-labeling, cells were co-cultured for 72 h at 2:1 Tcon:Treg ratio (1×105:5×104) in 96-well U-bottom plates pre-coated with 10 μg/mL anti-CD3 mAb (145.2C11; Tonbo Bioscience) or isotype control (Armenian hamster IgG isotype, BioLegend) plus 1 μg/mL soluble anti-CD28 (37.51; Tonbo Bioscience). Tconv cell proliferation was analysed by determining the percentage of CFSElow cells in a BD FACSCanto II flow cytometer.
Simões I.T., Aranda F., Carreras E., Andrés M.V., Casadó-Llombart S., Martinez V.G, & Lozano F. (2017). Immunomodulatory effects of soluble CD5 on experimental tumor models. Oncotarget, 8(64), 108156-108169.
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2

Treg Cell Induction from CD6 Knockout Mice

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Sorted Tconv cells (105 cells/well) from CD6−/− and CD6+/+ spleens were cultured for 3 days in U-bottomed 96-well plates coated with anti-CD3 mAb (145-2C11; 2 µg/mL, TONBO Bioscience) plus soluble anti-CD28 mAb (37.51; 5 µg/mL, TONBO Bioscience) in the presence of TGF-β (2 ng/mL, ImmunoTools) and IL-2 (20 ng/mL, ImmunoTools). Cells were then stained with anti-CD4 (FITC-RM4-5), anti-CD25 (APC-PC61.5), and anti-FoxP3 (PE-3G3, eBioscience) for further flow cytometry analysis of Treg (CD4+CD25+FoxP3+) cell induction.
Consuegra-Fernández M., Martínez-Florensa M., Aranda F., de Salort J., Armiger-Borràs N., Lozano T., Casares N., Lasarte J.J., Engel P, & Lozano F. (2017). Relevance of CD6-Mediated Interactions in the Regulation of Peripheral T-Cell Responses and Tolerance. Frontiers in Immunology, 8, 594.
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