Axiocam ccl camera

Mouse aortas were perfused with PBS followed by 10% formalin or 4% paraformaldehyde. Aortic root sections were embedded in OCT for frozen sectioning. Photos of aortic root sections were taken with an Axiovert 35 Zeiss microscope (Zeiss, Germany) equipped with an Axiocam CCl camera at × 25 magnification. En face stained aortas and light microscopy was obtained with a Nikon Stereo microscope equipped with a Spot Insight camera (Spot Imaging) at × 5 or × 10 magnification. Aortic root sections and en face preparations were stained with Oil Red-O in 60% isopropanol or 80% methanol. Aortic roots were additionally stained with haematoxylin and eosin or trichrome by the UMASS morphology core, with rat anti-mouse CD68 (1:200) (AbD Serotec, clone FA-11) and Cy3-smooth muscle actin (Sigma S6198) (1:200), or with anti-ICAM-1 BBIG-I1 (1:200) or VCAM-1 BBIG-V1 (1:200) (R&D systems) and rat anti-mouse CD31 (1:400) (BD) and mounted in Prolong Gold with DAPI (Life Technologies). Images were quantified using ImageJ or Image Pro Plus Analysis Software (Media Cybernetics).

Livers were isolated and fixed in 10% formalin, paraffin embedded, and stained with hematoxylin and eosin (H&E) or frozen in OCT and stained with Oil-Red-O. Images were taken with an Axiovert 35 Zeiss microscope (Zeiss, Germany) equipped with an Axiocam CCl camera at 10x or 20x magnification.